摘要
根据GenBank上已发表链球菌cps2J毒力基因序列设计合成了一对可扩增长度为1 152 bp目的片段的引物,成功建立了特异性PCR方法用于食源性溶血链球菌毒力基因cps2J的检测。该方法的特异性和灵敏性试验结果显示,对大肠杆菌、沙门氏菌、金黄色葡萄球菌、霍乱弧菌、副溶血性弧菌、绿脓杆菌、粪肠杆菌、变形杆菌以及藤黄微球菌的PCR检测结果均呈阴性,对分离的9株食源性溶血链球菌菌株进行了检测,电泳结果均呈阳性;检测目的菌株DNA的最低敏感度可达3.2×10^(-1)ng/m L。结果表明,此法特异性强,灵敏性高,能准确地检测肉制品中携带毒力基因cps2J的溶血链球菌,可作为食源性溶血链球菌快速诊断和流行病学调查的手段。
A pair of primers with a length of 1 152 bp were designed and synthesized according to the virulence gene sequence of Streptococcus cps2J virulence gene released from GenBank, and a specific PCR assay was successfully established to detect cps2J virulence gene of a foodbome hemolytic Streptococcus. The specificity and sensitivity test of this method showed that PCR determination for Escherichia coli, Salmonella, Staphylococcus aureus, Vibrio cholerae, Vibrio parahaemolyticus, Pseudomonas aeruginosa, Fenchangganjun, Proteus and Enterococcus Micrococcus luteus were negative, but positive for 9 strains of foodbome Streptococcus. The lowest sensitivity for detection of strain DNA could reach 3.2×10^-1 ng/mL. In conclusion, this method could be used to detect the virulence gene cps2J of foodbome Streptococcus in meat product with its strong specificity and high sensitivity and could be used as a rapid diagnostic and epidemiological investigation method for foodbome Streptococcus.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2017年第7期2880-2883,共4页
Genomics and Applied Biology
基金
广西科技重大专项计划项目(桂科重14121003-4-1)资助
