摘要
目的建立灵芝孢子油HPLC指纹图谱并鉴别其油类混淆品。方法采用HPLC,色谱柱为ACCHROM Unitary-C_(18)(250 mm×4.6 mm,5μm),流动相为乙腈-异丙醇(51︰49),流速为1.0 mL·min^(-1),柱温为30℃,以蒸发光散射检测。结果建立了灵芝孢子油脂肪酸酯类成分HPLC指纹图谱,确定了8个共有峰,分别为三亚油酸甘油酯、1,2-二亚油酸-3-油酸甘油酯、1,2-二亚油酸-3-棕榈酸甘油酯、1,2-二油酸-3-亚油酸甘油酯、1-棕榈酸-2-油酸-3-亚油酸甘油酯、三油酸甘油酯、1,2-二油酸-3-棕榈酸甘油酯、1,2-二油酸-3-硬脂酸甘油酯。灵芝孢子油混淆品的图谱与灵芝孢子油指纹图谱有明显区别。结论建立的灵芝孢子油脂肪酸酯类成分指纹图谱能很好地区别灵芝孢子油和其混淆品。
OBJECTIVE To establish a specific HPLC fingerprint of the oil of Ganoderma lucidum spore and apply for identifying for its confusion. METHODS The HPLC chromatography was performed on an ACCHROM Unitary-C_(18) column(250 mm×4.6 mm, 5 μm) with the mobile phase of acetonitrile-isopropanol(51︰49). The flow rate was 1.0 mL·min^(-1) at 30 ℃with the detection of ELSD. RESULTS The chromatographic fingerprint of aliphatic esters of oil of Ganoderma lucidum spore was established. The fingerprint contained 8 recognizable peaks of trilinolein, 1,2-linolein-3-olein, 1,2-linolein-3-palmitin, 1,2-olein-3-linolein, 1-palmitin-2-olein-3-linolein, triolein, 1,2-olein-3-palmitin and 1,2-olein-3-stearin. The fingerprint of oil of Ganoderma lucidum spore was different from its confution obviously. CONCLUSION The established fingerprint can distinguish oil of Ganoderma lucidum spore from its confusion.
出处
《中国现代应用药学》
CAS
CSCD
2017年第7期1011-1014,共4页
Chinese Journal of Modern Applied Pharmacy
基金
浙江省食品药品监管系统科技计划(2014003)