摘要
目的研究黄芪甲苷对糖尿病模型大鼠造影剂肾损伤的保护作用,并从炎性因子、抗氧化应激等方面探讨黄芪甲苷对造影剂肾损伤的保护机制。方法 Wistar大鼠70只,取10只设为正常对照组、其余大鼠以链脲佐菌素制备糖尿病大鼠模型。造模次日将大鼠随机分为模型对照组、阳性对照组[厄贝沙坦50 mg·(d·kg)^(-1)]、造影剂损伤组和黄芪甲苷低、中、高[2.5、5、10mg·(d·kg)^(-1)]3个剂量组[5],均一次性给予60%泛影葡胺10ml·kg^(-1)尾静脉注射。给药组每天灌胃给药1次,正常对照组、模型对照组、造影剂损伤对照组均灌胃给予等体积生理氯化钠溶液,每天1次,连续21d。比较各组大鼠血糖、肾脏肥大指数、尿素氮(BUN)、血肌酐(Cr)、尿微量白蛋白(UmAlb)、尿α1-微球蛋白(Uα1-MG)水平、24h尿蛋白(UP24)等肾功能相关指标,比较各组血清白细胞介素-6(IL-6)水平,血清超氧化物歧化酶(SOD)活性、脂质过氧化代谢产物丙二醛(MDA)含量的改变,观察肾脏病理显微结构。结果模型组大鼠血糖维持在较高水平、肾脏肥大指数、BUN、Cr、UP24、UmAlb、Uα1-MG水平、血清IL-6较正常组明显升高(P<0.01),血清SOD活性低下,MDA含量增加(P<0.01)。造影剂损伤组较模型组组各项指标均进一步恶化。黄芪甲苷干预组与造影剂损伤组相比,肾脏肥大指数、BUN、Cr、UP24、UmAlb、Uα1-MG、IL-6水平明显改善(P<0.01),血清SOD活性增强,MDA含量减少(P<0.01)。结论对糖尿病造影剂肾损伤大鼠,黄芪甲苷在改善肾功能基本指标的同时,可能通过降低炎性因子血清IL-6水平、提高机体抗氧化能力发挥其肾功能的保护作用。
Objective To investigate the effect of astragaloside IV （AsIV） on IL6 and anti-oxidative stress in rats with diabetic radiocontrast media-induced nephropathy（RCIN）.Methods Seventy Wistar rats were randomly divided into seven groups（ n = 10） ：the normal control group, modlel control group,positive control group,radiocontrast media-induced injury group and low,middle and high dose of As IV groups.As IV 10,5,2.5 mg·（d·kg）^-1 was given to the rats in As IV groups for 21 days,while the other groups were given the same volume of physiological saline.The fasting blood glucose （FBG）, kidney weight/body weight, blood urea nitrogen, serum creatinine, and urine protein 24 h were measured by an au- tomatic biochemical analyzer and urine microalbumin, Urinary al-microglobulin, IL-6, SOD, and MDA were detected by different methods, while the physiological microstructure of the kidney was observed.Results FBG, kidney weight/body weight, BUN, Cr, UP24, UmAlb and Uα1-MG IL-6 were significantly increased in the model group compared with the nor mal group（ P 〈0.01）.Serum SOD activity was significantly decreased and MDA content was increased in the model group rats compared with the normal group（ P 〈0.01）.Each index further deteriorated in the radiocontrast mediainduced injury group compared with the model control group. Kidney weight/body weight, BUN, Cr, UP24, UmAlb and Uα1-MG IL-6 were significantly ameliorated in As IV groups compared with the radiocontrast media-induced injury group（ P 〈0.01）. Serum SOD activity significantly increased and MI）A content decreased in the As IV group rats compared with the model group（ P 〈0.01）.Renal histological analysis indicated granulovacuolar deneneration, brush border thinning and exfoliation in tubular cells in radiocontrast media-induced injury group rats.Conclusion As IV could improve renal function in rats with diabetic RCIN by reducing IL-6 and enhancing anti-oxidative capacity.
出处
《解放军药学学报》
CAS
CSCD
2017年第3期218-221,共4页
Pharmaceutical Journal of Chinese People's Liberation Army
关键词
黄芪甲苷
造影剂肾病
泛影葡胺
糖尿病肾病
白介素-6
氧化应激
大鼠
astragaloside IV
radiocontrast media-induced nephropathy
diatrizoate
diabetic nephropathy
IL-6
oxidative stress
rats
