摘要
目的探讨二肽基肽酶-4(DPP-4)和白介素-6(IL-6)在热性惊厥(FS)疾病中的表达变化及意义。方法选取SPF级正常14日龄SD大鼠,通过经典FS热水浴模型进行造模。利用大鼠全基因组表达谱芯片筛选差异基因,并利用生物信息学方法进行相互作用网络分析,采用荧光定量PCR(QPCR)、蛋白免疫印迹法(WB)、酶联免疫吸附方法(ELISA)检测2组DPP-4和IL-6的基因和蛋白表达水平。选取50例FS患儿为热性惊厥组和25例健康体检儿童为正常对照组,采用QPCR和ELISA检测2组DPP-4和IL-6基因及蛋白表达水平。结果表达差异基因相互作用网络图显示免疫因子DPP-4与IL-6之间可能存在相互作用关系;动物及临床水平表明,FS组DPP-4及IL-6基因表达水平显著高于正常组,差异均有统计学意义(P<0.05)。结论 DPP-4及IL-6基因及蛋白在动物及临床水平具有高表达模式,提示这两个基因与FS的发生发展存在密切联系,揭示了免疫因素在FS发生发展中起到非常重要作用,为研究FS的发病机制提供了新的临床依据和新的治疗靶点。
Objective To investigate the expression and significances of DPP-4 and IL-6 in febrile seizures （FS）. Methods FS were induced in Sprague-Dawley（SD） rats at P14 in a hot water bath by using classical model of hyperthermia-induced seizures. A genome-wide microarray experiment was generated in the rats. The relationship between the differentially expressed genes were analyzed by the method of bioinformatics, and the gene and protein levels of DPP-4 and IL-6 were detected by QPCR,WB and ELISA. Selected 50 children with FS（ FS group） and 25 healthy children（ control group） , and to compare the gene and protein levels of DPP-4 and IL-6 between the two groups. Re- suits Interaction network diagram of differential gene expression showed that there may be interactions between DPP-4 and IL-6. Animal and clinical experiments showed that the DPP-4 and IL-6 gene and protein levels were significantly higher in FS group compared with control group （ P 〈 0.05 ）. Conclusion There were high gene and protein expressions of DPP-4 and IL-6 in the FS group compared with the control group. These results indicated that immune and inflammations may play an important role in the FS, and it has provided an attractive pharma- cological target for the treatment of FS in clinic.
作者
徐健
高健
张成元
范元腾
XU Jian GAO Jian ZHANG Cheng-yuan FAN Yuan-teng(Department of Laboratory,Weifang Maternity and Child Hospi- tal, Weifang Shandong 261011, China Department of Pediatrics, Weifang Maternity and Child Hospital, Weifang Shandong 261011, China Neonate Department, Weifang Maternity and Child Hospital, Weifang Shandong 261011, China Department of Pathophysiology, School of Basic Medical Sciences,Wuhan University,Wuhan Hubei 430071, China)
出处
《局解手术学杂志》
2017年第8期568-572,共5页
Journal of Regional Anatomy and Operative Surgery
基金
潍坊市卫生局科研项目计划(2014002)
