摘要
目的探讨生殖道支原体对米诺环素的耐受性及其相关耐药基因携带情况。方法分别从1 076例生殖道感染者的生殖道分泌物中分离得到生殖道支原体,通过支原体鉴定药敏试剂盒鉴定支原体及其对米诺环素的敏感性,然后采用耐药基因检测试剂盒检测各菌株四环素耐药基因TetM的阳性率。结果共分离得到387株支原体阳性菌株,其中解脲支原体(UU)占71.06%,人型支原体(MH)占5.43%,UU+MH占23.51%;随着米诺环素浓度增大,菌株耐受性逐渐降低,当米诺环素浓度为1.000 0mg/L时抑菌效果最佳;TetM基因在中介菌株检出率显著高于敏感菌株,差异有统计学意义(P<0.05);TetM基因在耐药菌株中的阳性检出率最高,显著高于中介菌株、敏感菌株,差异均有统计学意义(P<0.05)。结论生殖道支原体对米诺环素米诺环素有一定耐受性,TetM基因与菌株米诺环素耐受性密切相关,可以作为判断米诺环素耐药性的重要参考依据。
Objective To explore the resistance of genital Mycoplasma to minocycline and the distribution of related resistant gene. Methods Genital Mycoplasma was isolated from 1 076 genital tract infections. Immu-Mark Myco-Test Kit was used to identi- fy Mycoplasma and their sensibilities to tetracyclines were dectected. Then positive rate of Mycoplasma with minocycline-resistance gene TetM through Kit was investigated. Results A total of 387 strains of Mycoplasma positive strains were isolated,UU strains were accounted for 71.06% ,MH strains were accounted for 5.43% ,UU＋MH strains were accounted for 23.51%. With the in- crease of the concentration of minocycline, strain tolerance was decreased gradually,when minocycline concentration was 1. 000 0 mg/L,the antibacterial effect was the best. The detection rate of TerM gene in intermediate strains was significantly higher than that of the susceptible strain（P〈0.05）. The positive detection rate of TetM in the drug resistant strains was significantly higher than that of the intermediate strains and the sensitive strains（P〈0.05）. Conclusion Genital Mycoplasma strains have shown var- ying degrees of tetracycline minocycline resistance. Tetracycline-resistance of strains is closely related to TetM gene, which could be an important reference to assess the tetracycline resistance of Mycoplasma infections.
作者
谢明水
刘杨
XIE Mingshui L IU Yang(Department of Clinical Laboratory, Suizhou Hospital Affiliated of Hubei University of Medicine, Suizhou, Hubei 441300, China)
出处
《检验医学与临床》
CAS
2017年第15期2264-2265,2324,共3页
Laboratory Medicine and Clinic
