摘要
目的:研究连翘苷对环磷酰胺处理小鼠免疫功能的影响。方法:以腹腔注射环磷酰胺法复制小鼠免疫抑制模型,试验组小鼠分别按50、100、150 mg/kg剂量灌胃连翘苷,同时设模型组和对照组(等体积生理盐水灌胃),连续处理7 d。常规方法测定胸腺和脾的脏器系数,FITC微球法测定腹腔巨噬细胞吞噬功能,ELISA法检测外周血IL-2、IL-4和外周血淋巴细胞c AMP含量,分析不同剂量的连翘苷对免疫抑制小鼠上述指标的影响。结果:7 d后成功建立免疫抑制小鼠模型。连翘苷能够不同程度地提高免疫抑制小鼠的免疫器官指数、巨噬细胞吞噬能力和血清中免疫因子含量,降低外周血淋巴细胞中c AMP含量。结论:连翘苷能够抵抗环磷酰胺的免疫抑制作用,具有一定的免疫调节功能。
Objective：To study the effect of phillj^rin on immune function in mice treated with cyclophosphamide. Methods： Mice model of immunosuppression was induced by intraperitoneal injection of cyclophosphamide,the mice in the experimental group received phillyrin with 50,100 and 150 mg/kg doses by gavage. While model group and control group received normal salin,all treatments continued 7 d. The thymus and spleen organ coefficients was measured by conventional methods. The phagocytosis of peritoneal macrophages was measured by FITC microspheres. The levels of IL-2, IL^ and cAMP in peripheral blood were detected by ELISA. The effects of different doses of phillyrin on the above indexes were analyzed in immunosuppressed mice. Results ： A mice model of immunosuppression was established after 7 d, the results showed that the immune organ index, phagocytic capacity of macrophages and the level of immune factors in serum could be improved,and the content of cAMP in peripheral blood lymphocytes was decreased in different degree by phillyrin. Conclusion： Phillyrin has the function of immune regulation,which can resist the immuno-suppression of cyclophosphamide.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2017年第8期1177-1180,共4页
Chinese Journal of Immunology
基金
辽宁省科技厅2014年自然科学基金项目(No.2014022048)
关键词
连翘苷
小鼠
免疫抑制
Phillyrin
Mice
Immunosuppression
