摘要
目的建立HPLC-DAD法同时测定复方罗布麻片Ⅰ(罗布麻叶、野菊花、防己等)中4种成分的含有量。方法该药物50%甲醇提取液的分析采用Shimadzu VP-ODS色谱柱(250 mm×4.6 mm,5μm);流动相乙腈-0.5%磷酸,梯度洗脱;体积流量1.0 m L/min;柱温35℃;检测波长260、325 nm。结果绿原酸、氢氯噻嗪、蒙花苷、盐酸异丙嗪分别在24.91~498.2 ng(r=0.999 9)、286.33~5 726.7 ng(r=0.999 9)、10.04~200.9 ng(r=0.999 9)、154.80~3 096.1 ng(r=0.999 9)范围内呈良好的线性关系,平均加样回收率分别为98.3%(RSD=1.3%)、99.1%(RSD=0.6%)、98.5%(RSD=1.0%)、99.3%(RSD=1.2%)。结论该方法简便、准确、可靠,可用于复方罗布麻片Ⅰ的质量控制。
AIM To establish an HPLC-DAD method for the simultaneous content determination of four constituents in Compound Kendir Leaves TabletsⅠ（ Apocyni veneti Folium,Chrysanthemi indici Flos,Stephaniae tetrandrae Radix,etc.）. METHODS The analysis of 50% methanol extract of this drug was performed on a 35 ℃thermostatic Shimadzu VP-ODS column（ 250 mm × 4. 6 mm,5 μm）,with the mobile phase comprising of methanol-0. 5% phosphoric acid flowing at 1. 0 m L/min in a gradient elution manner,and the detection wavelengths were set at 260 nm and 325 nm. RESULTS Chlorogenic acid,hydrochlorothiazide,buddleodide and promethazine hydrochloride showed good linear relationships within the ranges of 24. 91-498. 2 ng（ r = 0. 999 9）,286. 33-5 726. 7 ng（ r = 0. 999 9）,10. 04-200. 9 ng（ r = 0. 999 9） and 154. 80-3 096. 1 ng（ r = 0. 999 9）,whose average recoveries were 98. 3%（ RSD = 1. 3%）,99. 1%（ RSD = 0. 6%）,98. 5%（ RSD = 1. 0%） and 99. 3%（ RSD = 1. 2%）,respectively. CONCLUSION This simple,accurate and reliable method can be used for the quality control of Compound Kendir Leaves TabletsⅠ.
出处
《中成药》
CAS
CSCD
北大核心
2017年第8期1625-1628,共4页
Chinese Traditional Patent Medicine
关键词
复方罗布麻片Ⅰ
绿原酸
蒙花苷
氢氯噻嗪
盐酸异丙嗪
HPLC-DAD
Compound Kendir Leaves Tablets Ⅰ
chlorogenic acid
buddleodide
hydrochlorothiazide
promethazine hydrochloride
HPLC-DAD
