摘要
目的检测微小RNA-218(microRNA-218,miR-218)在乳腺癌组织中的表达情况,并探讨其临床意义。方法收集45例乳腺癌手术标本及其对应的癌旁正常组织。采用real-time qPCR检测组织中miR-218的表达,分析乳腺癌组织与癌旁组织中miR-218的表达水平以及其与患者临床病理参数之间的关系。结果乳腺癌组织中miR-218的相对表达量明显低于癌旁正常组织(P=0.001)。PR阴性的乳腺癌组织中miR-218的表达水平明显低于PR阳性乳腺癌组织(P=0.037)。Ki-67阳性的乳腺癌组织中miR-218的表达水平明显低于Ki-67阴性的乳腺癌组织(P=0.018)。结论 miR-218与乳腺癌的发生、临床进展及预后密切相关,它可能为我们提供乳腺癌新的早期发现、早期治疗方法及判断预后、监测治疗效果的分子标志物。
Objective To investigate the expression of miR-218(microRNA-218)in breast cancer tissues and its clinical significance.Methods Totally 45 tissue biopsies gained from breast cancer patients and the adjacent normal tissue were collected.Real-time qPCR was used to detect the miR-218 expressions.The correlations of miR-218 expression with clinicopathological characteristics and prognosis of breast cancer patients were analyzed.Results The average expression level of miR-218 in breast cancer tissues was significantly lower than that in control tissues(P=0.001).The average expression level of miR-218 in PR negative breast cancer tissues was significantly lower than that of PR positive breast cancer tissue(P=0.037).The average expression level of miR-218 in Ki-67 positive breast cancer tissues was significantly lower than that in Ki-67 negative breast cancer tissue(P=0.018).Conclusion The expression of miR-218 is closely related to carcinogenesis,progression and prognosis of breast cancer,so it may be served as a diagnostic biomarker and a prognostic predictor in breast cancer patients,which provides a new way for the treatment of breast cancer.
出处
《实用医学杂志》
CAS
北大核心
2017年第15期2508-2511,共4页
The Journal of Practical Medicine
基金
河北省科技计划项目(编号:14277755D)
