摘要
目的:建立复方枇杷喷托维林颗粒中甘草酸的鉴别和含量测定方法。方法:采用薄层色谱对甘草酸进行定性鉴别;采用高效液相法测定甘草酸含量,使用Techmate C_(18)(250 mm×4.6 mm,5μm)色谱柱;流动相为甲醇-0.2 mol·L^(-1)醋酸铵-冰醋酸(60∶39∶1);流速:1.0 ml·min^(-1);检测波长:250 nm,柱温:35℃;进样量:20μl。结果:薄层鉴别斑点清晰,阴性对照无干扰。在选定的色谱条件下,甘草酸在0.01~1.01 g·L^(-1)的质量浓度范围内线性关系良好,r=1.000 0,平均加样回收率103.2%,RSD=1.8%(n=9)。结论:本方法简便、准确,可用于该制剂的质量控制。
Objective： ： To establish a quality standard for glycyrrhizic acid in compound loquat and pentoryverine granule. Meth- ods： TLC was applied in the qualitative detection of glycyrrhizic acid. An HPLC method was used for the quantitative determination of glycyrrhizic acid. A C,s （250 mm ×4.6 mm,5μm） column was used. The mobile phase was a mixture of methanol-0.2 mol L-1 ammonium acetate-acetic acid（60 ： 39 ： 1 ）. The flow rate was at 1.0 ml min-1, and the detection wavelength was at 250 nm. Results： The TLC spots were clear and well＂ separated without interference from the negative sample. The calibration curves were linear within the range of 0.01-1.01 g L-1 （r= 1.000 0）. The average recovery was 103.2% （RSD% = 1.8%, n =9）. Conclusion： The method is simple and accurate, which can be used for the quality control of compound loquat and pentoryverine granule.
出处
《中国药师》
CAS
2017年第8期1461-1463,共3页
China Pharmacist
基金
国家药典委员会2015年标准提高研究项目(编号:249)
