摘要
目的:建立止咳祛痰口服液的质量标准。方法:采用TLC法对桔梗、甘草进行定性鉴别;采用HPLC法对射干的有效成分射干苷进行定量测定,色谱柱为Waters Symmetry C_(18)柱(150 mm×4.6 mm,3.5μm),流动相为乙腈-水(14∶86),流速1.0 ml·min^(-1),柱温35℃,检测波长为265 nm。结果:薄层色谱斑点清晰,阴性无干扰。射干苷在0.115~2.880μg范围内线性关系良好(r=0.999 9),平均回收率为92.44%,RSD为1.83%(n=6)。结论:所建立的方法简便、快速、重复性好,可用于止咳祛痰口服液的质量控制。
Objective： To establish the quality standard for Zhike Qutan oral liquid. Methods： A TLC method was used for the qualitative identification of Platycodonis Radix and Glycyrrhizae Radix et Rhizoma; the content of belamcandin in Belamcandae Rhizoma was determined by an HPLC method on a Waters Symmetry C,s column（ 150 mm×4.6 mm,3.5μm）with the mobile phase consisting of acetonitrile-water （ 14 ： 86）, the flow rate was 1.0 ml min -1, the column temperature was 35℃ and the detection wavelength was 265 nm. Results ： The TLC spots were clear without interference from the negative control. The linear range of belamcandin was 0.115- 2.880 μg （r =0.999 9）,and the average recovery was 92.44% （RSD = 1.83%, n = 6）. Conclusion： The method is simple and rapid with good reproducibility, which can be used for the quality control of Zhike Qutan oral liquid.
出处
《中国药师》
CAS
2017年第8期1511-1513,共3页
China Pharmacist
关键词
止咳祛痰口服液
桔梗
甘草
射干苷
质量标准
薄层色谱
高效液相色谱
Zhike Qutan oral liquid
Platycodonis Radix
Glycyrrhizae Radix et Rhizoma
Belamcandin
Quality standard
TLC
HPLC
