摘要
目的对四川省人民医院肝脓肿患者血培养分离肺炎克雷伯杆菌铁系统基因携带情况进行分析。方法收集2015年1月至12月四川省人民医院检验科保存的10株来源于肝脓肿患者血培养分离的肺炎克雷伯杆菌,提取细菌基因组,通过PCR法测定肺炎克雷伯杆菌iroB、iroC、iroD基因情况,并利用Graphpad Prsim 5.0软件对结果进行分析。结果 10株血培养来源肺炎克雷伯杆菌株中,iroB、iroC、iroD基因阳性的菌株分别有10株、9株和9株,其中iroB/C/D基因均阳性的菌株为9株。结论肝脓肿患者肺炎克雷伯杆菌铁系统基因iroB/C/D阳性率高,同时携带iroB/C/D基因可能为该菌株感染引起肝脓肿的重要因素。
Objective To investigate the iron regulated locus in Klebsiella pneumoniae isolates from blood culture of liver abscess patients in Sichuan Provincial People＇s Hospital. Methods From January to December of 2015, a total of 10 isolates of Klebsiella pneumoniae were collected from blood culture of liver abscess patients from Sichuan Provincial People＇s Hospital. The genomic DNA was extracted to identify the genes of iroB, iroC, and iroD by PCR, and data was further analyzed by Graphpad Prism 5 software. Results Among the 10 Klebsiella pneumoniae clinical strains, 9 strains were iroB positive strains, 9 strains were iroC positive strains, and 10 strains were iroD positive strains, 9 strains were iroB/C/D triple positive. Conclusion The current study suggests that the frequency of triple positive of iroB/C/D in Klebsiella pneumoniae is high in isolates from liver abscess patients, the triple positive of iroB/C/D may contribute to liver abscess.
出处
《中国循证医学杂志》
CSCD
2017年第8期907-909,共3页
Chinese Journal of Evidence-based Medicine
基金
四川省卫生计生委项目(编号:17PJ509)
四川省科技支撑计划项目(编号:2014SZ0053)
关键词
肺炎克雷伯杆菌
铁系统基因
肝脓肿
危险因素
Klebsiella pneumoniae
Iron regulated locus
Liver abscess
Risk factor
