摘要
目的研究鸢尾素对载脂蛋白E基因敲除(ApoE^(-/-))小鼠动脉粥样硬化的影响。方法将ApoE^(-/-)小鼠随机分为正常对照组、动脉粥样硬化模型组、鸢尾素组,每组10只,分别给予普通饮食+生理盐水、高脂饮食+生理盐水、高脂饮食+鸢尾素,饲养至12周后处死,处死前测体重,用化学法测定总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDLC)、高密度脂蛋白胆固醇(HDLC)、血清超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量。采用HE染色和整体油红O染色分析主动脉粥样斑块面积大小,实时荧光定量(qRT-PCR)检测主动脉CD36、NF-κB p65的mRNA表达,Western blot检测主动脉CD36、NF-κB p65、SOD的蛋白表达。结果动脉粥样硬化模型组主动脉斑块面积明显高于正常对照组,内膜明显增厚,而鸢尾素组较动脉粥样硬化模型组斑块面积减少,内膜增厚程度下降。与正常对照组比较,动脉粥样硬化模型组小鼠体重、TC、TG、LDLC、MDA水平升高,NF-κB p65、CD36mRNA和蛋白表达增加,而血清SOD活性、HDLC水平减低(P<0.05)。与动脉粥样硬化模型组比较,鸢尾素组小鼠体重、TC、TG、LDLC、MDA水平降低,NF-κB p65、CD36 mRNA和蛋白表达下调,而血清SOD活性、HDLC水平升高(P<0.05)。结论鸢尾素能减轻ApoE^(-/-)小鼠动脉粥样硬化的发生发展,这可能与其降低小鼠体重和血脂、抑制炎症反应、抗氧化应激有关。
Aim To study the effect of irisin on the process of atherosclerosis in apolipoprotein E knockout（ ApoE^（-/-）） mice. Methods ApoE^（-/-）mice were randomly divided into three groups： normol group,atherosclerosis model group and irisin group,each group contained 10 mice. ApoE^（-/-）mice in normol group were fed with normal diet and saline,ApoE^（-/-）mice in atherosclerosis model group were fed with high fat diet and saline,ApoE^（-/-）mice in irisin group were fed with high fat diet and irisin,all mice were fed for 12 weeks. Total cholesterol（ TC）,triglyceride（ TG）,low density lipoprotein cholesterol（ LDLC）,high density lipoprotein cholesterol（ HDLC）,the serum levels of superoxide dismutase（ SOD） and malondialdehyde（ MDA） were measured by chemical method,HE staining and total oil red O staining were used to analyze the size of aortic atherosclerotic plaque area,the mRNA and protein level of CD36,NF-κB p65,SOD were detected by real-time quantitative PCR（ q PCR） and Western blot. Results The area of aortic plaque in atherosclerosis model group was significantly higher than that in normal group,the area of aorta plaque in irisin group was significantly lower than that in atherosclerosis model group. Compared with normal group,body weight of mice,TC,TG,LDLC,MDA,the expression of NF-κB p65 and CD36 mRNA and protein in atherosclerosis model group increased,while the activity of serum SOD and HDLC levels decreased（ P〈0.05）. Compared with atherosclerosis model group,body weight of mice,TC,TG,LDLC,MDA,the expression of NF-κB p65 and CD36 mRNA and protein in irisin group decreased,while the activity of serum SOD and HDLC levels increased（ P〈0.05）. Conclusion Irisin can reduce the occurrence and development of atherosclerosis in ApoE^（-/-）mice,which may be related to its decreasing body weight and blood lipid,inhibition of inflammation and anti-oxidative stress.
出处
《中国动脉硬化杂志》
CAS
北大核心
2017年第8期773-777,共5页
Chinese Journal of Arteriosclerosis
基金
国家自然科学基金项目(81660083)
贵州省高层次创新型人才培养计划项目[黔科合人才(2015)4026号]
贵州省社会发展攻关项目[黔科合SY(2013)3017号]
贵州省优秀青年科技人才培养对象专项资金项目[黔科合人字(2011)26号]
贵州省对外合作项目[黔科合省合(2011)7001号]
