摘要
目的探讨神经节苷脂对脑出血（ICH）大鼠脑组织的保护作用机制。方法取健康SD大鼠185只，随机分成假手术组（A组）、脑出血组（B组）和神经节苷脂干预组。通过立体定位仪诱导脑出血模型。各组大鼠于不同时间点进行神经功能缺损评分并进行采样，观察大鼠脑含水量、诱导型一氧化氮合酶（iNOS）和细胞间黏附分子-1（ICAM-1）表达的变化情况。结果①大鼠发生脑出血后，24h神经功能缺损达到高峰，72h脑含水量达到高峰值，与A组相比，B组神经功能缺损和脑含水量均增加（P值均〈0．05）。与B组相比，神经节苷脂干预组能减轻神经功能缺损、降低脑含水量（P值均〈0．05）。②大鼠发生脑出血后，血肿周边区iNOS和ICAM-1的表达水平均升高，出血后72h二者的表达达到高峰，与B组相比，神经节苷脂干预组能显著降低iNOS及ICAM-1表达的水平（P值均〈0．05），且在实验选取的干预剂量中，30mg／（kg·d）比15mg/（kg·d）和60mg／（kg·d）效果更好（P〈0．05）。结论神经节苷脂能减少脑出血后脑含水量及减轻继发性神经功能缺损。降低INOS和ICAM-1的表达水平可能是神经节苷脂减轻脑出血急性期脑水肿的机制之一。
Objective To observe the brain protective effect and its mechanism of gangliosides in intracerebral hemorrhage rats, and to explore the new approach in treatment of cerebral hemorrhage. Methods The 185 healthy Sprague Dawley （SD） rats were randomly selected and divided into shamed- operation group （A group ）, intracerebral hemorrhage group （B group）, ICH gangliosides therapy. Rats were observed by neurological deficits and executed at each point, and brain water content, iNOS and ICAM - 1 in rats＇ brain of intracerebral hemorrhage model group were analyzed. Results （1）After intracerebral hemorrhage happened in rats, neurological deficits reached peak at 24 h time point, and brain water content reached peak at 72 h time point. Neurological deficit and brain water content in group B showed significant difference with group A （ P 〈 0.05 ）. In comparison with B group, neurological deficits and brain water content of group C, D and E were significantly reduced （ P 〈 0.05 ）. （2）After intracerebral hemorrhage happened in rats, the expression of iNOS and ICAM - 1 reached peak at 72 h time point. In comparison with B group, the expression levels of iNOS and ICAM - 1 of group C, D and E were significantly reduced （P 〈 0. 05）; also, the selected dose in the experiment, 30 mg/kg showed better function than 15 mg/kg and 30 mg/kg （P 〈 0.05 ）. Conclusion Gangliosides may reduce the brain water content and improve the neurologic deficits of intracerebral hemorrhage. Gangliosides can reduce iNOS and ICAM - 1 expression, which may be the possible mechanism of its decreased brain edema effect.
出处
《中国急救医学》
CAS
CSCD
北大核心
2017年第8期746-749,I0002,共5页
Chinese Journal of Critical Care Medicine
基金
福建省科技厅自然科学基金面上项目（2012J01437）
宁德市科技计划项目（20120084）
关键词
脑出血(ICH)
脑水肿
神经节苷脂
机制
Intracerebral hemorrhage （ICH）
Brain edema
Gangliosides
Mechanism
