CD69+CD4+CD25-T在自身免疫性胰腺炎患者外周血中表达升高及临床意义被引量：2

Elevated Expression of CD69+CD4+CD25-T Cells in Peripheral Blood from Patients with Autoimmune Pancreatitis and Its Clinical Significance

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摘要目的检测CD69+CD4+CD25-T细胞在自身免疫性胰腺炎(AIP)患者外周血中的表达情况,进一步分析其临床意义。方法收集2014年9月~2016年12月长海医院血液科诊断的32例AIP患者与同期体检的32例健康个体外周血分别作为实验组与对照组。采用密度梯度离心法分离外周血单个核细胞(PBMCs),用流式细胞术检测PBMCs中的CD69+CD4+CD25-T,以ELISA法检测血浆中细胞因子的表达状况。两组间变量的比较采用两独立样本<检验,两计量资料间的关系以Pearson相关系数表示。结果实验组与对照组CD69+CD4+CD25-T表达水平为10.36%±3.68%vs3.99%±1.45%,(t=9.110,P<0.000 1);TGF-β的表达水平为399.86±121.88vs 143.87±56.22 pg/ml,(t=10.79,P<0.000 1),差异均具有统计学意义。实验组CD69+CD4+CD25-T细胞与TGF-β呈正相关(r=0.653,P<0.001),与IL-4,IFN-γ,IL-2呈负相关(r=-0.442,-0.567,-0.351,P<0.05),差异具有统计学意义。结论 CD69+CD4+CD25-T细胞可能参与了AIP的免疫病理过程,是临床诊断与治疗潜在的生物学指标。 Objective To test the expression level of CD69＋CD4＋CD25-T cells in peripheral blood from patients with auto- immune pancreatitis,and further analyze its clinical significance. Methods Peripheral blood samples from 32 patients with AIP diagnosed in hematological department, Changhai Hospital and 32 health individuals examined at the same time were collected from September 2014 to December 2016, they were classified as experimental and control groups, separately. Peripheral blood mononuclear cells （PBMCs） was acquired by density gradient centrifugation, CD69 ＋ CD4 ＋ CD25-T cells in PBMCs were tested by flow cytometry, and the expression level of cytokines in plasm was by ELISA. The comparison of varies between the two groups was measured by two independent samples＇ t test. The relationship between the two measure- ment data was measured by pearson correlation coefficient. Results The expression levels of CD69＋CD4＋CD25-T in experimental and control groups were 10.36%±3.68% vs 3.99%±1.45% （t=9. 110,P〈0. 0001）. The expression level of TGF-β was 399. 864-121.88 vs 143.87±56.22 pg/ml （t= 10.79,P〈0. 000 1） ,both with statistical significance. The levels of CD69 ＋ CD4 ＋ CD25-T in experimental was positively correlated with TGF-β （r= 0. 653, P〈0. 001） and negatively with IL-4,IFN-γ,IL-2 （r=-0. 442,-0. 567,-0. 351,P〈0.05） and there was statistical significance. Conclusion CD69＋CD4 ＋CD25-T cells might involve the immunopathology of AIP and could be the potential biomarker for clinical diagnosis and therapy.

作者李腾达龙曙萍黄元兰孙懿郭杰刘云张薇薇谷明莉邓安梅

机构地区第二军医大学长海医院检验科解放军

出处《现代检验医学杂志》 CAS 2017年第4期29-31,共3页 Journal of Modern Laboratory Medicine

基金 973计划(2013CB531606) 国家自然科学基金(81671556 81601406 81471605 81401358 81501397 31500721 81501398 81302579 81273282 81202353) 上海申康基金(SHDC22014014) 上海教育科学基金(D14017) 军队科研基金(BWS14J023 15ZD009 15XD007) 美捷登基金(MJR20150019)

关键词自身免疫性胰腺炎 CD69+CD4+CD25-T细胞转化生长因子-Β 白细胞介素-4 干扰素-Γ autoimmune pancreatitis CD69 ＋ CD4 ＋ CD25-T cells TGF-i3 IL-4 IFN-γ

分类号 R576 [医药卫生—消化系统] R392.11 [医药卫生—免疫学]

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参考文献1

1李腾达,刘挺挺,吴林洪,邓顺江,陈燕,谷明莉,邓安梅.原发性胆汁性肝硬化患者外周血单个核细胞中Tim-1表达增高及其意义[J].现代检验医学杂志,2016,31(4):41-43. 被引量：3

二级参考文献10

1Carey EJ,Ali AH,Lindor KD. Primary biliary cirrho- sis[J]. Lancet,2015,386(10003) :1565-1575.
2Fenoglio D, Bernuzzi F, Battaglia F, et al. Th17 and regulatory T lymphocytes in primary biliary cirrhosis and systemic sclerosis as models of autoimmune fi-brotic diseases [J ]. Autoimmunity Reviews, 2012, 12 (2) :300-304.
3Baier JL,Mattner J. Mechanisms of autoimmune liver disease[J]. Discovery Medicine, 2014 (18) : 255-263.
4Zhang XM, Shan NN, Sun M, et al. Imbalanced ex- pression of human Tim-1 and Tim-3 in peripheral blood mononuclear cells from immune thrombocyto- penia patients [J]. International Immunopharmacolo- gy,2014,19(1) :1-4.
5Li WX, Chen GM, Yuan H, et al. Polymorphisms of the TIM-1 and TIM-3 genes are not associated with systemic lupus erythematosus in a Chinese population [J]. Mutagenesis,2011,26(4) :507-511.
6Limongi F. Thl cytokines and chemokines in primary biliary cirrhosis[J]. La Clinica Terapeutica, 2015,166 (2): e122-125.
7Xu JR, Yang Y, Liu XM, et al. Polymorphisms of the TIM-1 gene are associated with rheumatoid arthritis in the Chinese Hui minority ethnic population[J]. Genet Mol Res,2012,11(1):61-69.
8Rennert PD. Novel roles for TIM-1 in immunity and infection[J]. Immunology Letters, 2011,141 ( 1 ) : 28- 35.
9Li Z,Ju Z, Frieri M. The T-ceU immunoglobulin and mucin domain(Tim) gene family in asthma,allergy, and autoimmunity[J]. Allergy Asthma Pro, 2013,34 (1) : e21-26.
10刘晓,黄元兰,胡志德,孙懿,陈燕,邓安梅,仲人前.原发性胆汁性肝硬化患者外周血单个核细胞中Tim-3 mRNA表达增高及其意义[J].现代免疫学,2012,32(4):299-302. 被引量：3