摘要
目的 :采用UPLC法测定11批不同产地五指毛桃中补骨脂素的含量,为其质量标准的建立提供一定参考依据。方法 :采用XBridge TM BEH C_(18)色谱柱(100 mm×4.6 mm,2.5μm),流动相:乙腈—水(40∶60),流速:0.5 m L/min,柱温:35℃,检测波长:245 nm。结果:补骨脂素对照品在0.78~78μg之间线性范围良好,线性回归方程Y=54.227X+90.378(r=0.9999),平均加样回收率为99.6%,RSD=2.05%(n=6)。各产地的五指毛桃含量在0.039%~0.070%之间,其中,含量排名前三的产地为:广东河源、广东从化、广东翁源,分别为0.070%、0.063%、0.058%。结论:不同产地五指毛桃的补骨脂素含量差异较大。UPLC测定方法出峰时间快,操作简单、准确,可为五指毛桃质量标准建立提供方法学支持。
Objective, To determine psoralen content in Ficus Hirta Vahl from eleven different habitats by UPLC （ultra performance liquid chromatography）, and to provide a reference for the establishment of its quality standard. Methods： XBridgeTM BEH C18 column （100 mm ×4.6 mm, 2.5 μm） was applied. The mobile phase was acetonitrile-water （40 ： 60）, flow rate was 0.5 mL/min, column temperature was 35℃, and wave length was 245 rim. Results： The reference substance of psoralen displayed good linear range between 0.78 and 78μg. The linear regression equation was Y=54.227X＋90.378 （r=0.9999） and the average recovery was 99.6%, RSD=2.05% （n=6）. The content of Ficus Hirta Vahl from various habitats ranged from 0.039% to 0.070%. Heyuan, Conghua and Wengyuan in Guangdong province were the first three habitats that had the highest content, being respectively 0.070%, 0.063%, 0.058%. Conclusion= Psoralen content in Ficus Hirta Vahl varies in different habitats. UPLC has advantages of fast peak time, simple and accurate operation. It can provide support for the establishment of quality standard of Ficus Hirta Vahl.
出处
《新中医》
CAS
2017年第8期16-19,共4页
New Chinese Medicine
基金
2015年广东省中医药局科研项目(20152144)
关键词
五指毛桃
补骨脂素
超高效液相色谱(UPLC)
含量测定
质量标准
Ficus Hirta Vahl
Psoralen
Ultra performance liquid chromatography （UPLC）
Content determination Quality standard
