摘要
对产Monacolin K的红曲霉菌株进行了复合诱变后的筛选和发酵条件优化。通过紫外-亚硝酸钠复合诱变,得到一株高产Monacolin K的菌株3.991-7。通过单因素试验,以Monacolin K的产量为指标,确定出最佳发酵条件:接种量7%,pH值为4.5,培养温度为25℃,培养时间为10 d。在此最佳条件下,诱变菌株固态发酵后的Monacolin K的产量为9.06 mg/g。
Monascus anka with Monacolin K yield was screened by compound mutagenesis, and the fermentation conditions was optimized. By UV and sodium nitrite compound mutation, a strain 3.991-7 with high yield Monacolin K was obtained. Using Monacolin K yield as index, the optimal fermentation conditions were optimized by single factor experiments as follows： inoculum 7%, pH 4.5, culture temperature 25 ℃, time 10 d. Under the conditions, the Monacolin K yield of mutant strain was 9.06 mg/g with solid-state fermentation.
作者
夏诗棋
管轶男
付正
邹艳
李俊杰
管斌
孔青
XIA Shiqi GUAN Yinan FU Zheng ZOU Yan LI Junjie GUAN Bin KONG Qing(College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China Qingdao Viand Biotech Inc., Qingdao 266101, China Shandong Medical College, Jinan 250002, China)
出处
《中国酿造》
CAS
北大核心
2017年第8期62-66,共5页
China Brewing
基金
"十二五"农村领域国家科技计划(013BAD10B02-06)
山东省科技计划项目(2014GSF121029)
关键词
红曲霉
莫纳可林K
发酵条件
复合诱变
Monascus anka
Monacolin K
fermentation conditions
compound mutation
