摘要
目的研究丙型肝炎病毒(HCV)基因分型,了解山东地区丙型肝炎的基因分型情况。方法收集448例丙型肝炎患者的血清标本,采用焦磷酸测序法对HCV感染标本进行分型。结果 448例丙型肝炎患者共检出7种基因亚型,分别为1b型288例(64.28%)、2a型137例(30.57%)、1a型13例(2.90%)、3b型4例(0.90%)、3a型2例(0.45%)、6a型2例(0.45%)、1a1b型2例(0.45%);对HCV基因型与性别、年龄的关系进行分析显示无明显相关性;2013-2016年各基因型患病率分析结果显示无明显的变化趋势。结论山东地区HCV感染基因型以1b型为主,其次为2a型;HCV基因型分布与性别、年龄差异无统计学意义(P>0.05),且近4年山东地区各基因型年患病率无明显变化趋势。
Objective To study the genotype of hepatitis C virus（HCV）and understand the genotype distribution of hepatitis C in Shandong area.Methods Serum samples from 448 patients with hepatitis C were collected and classified into HCV infected samples by pyrosequencing.Results Totally 448 cases of patients with hepatitis C were detected in 7genotypes,respectively,288 cases of type 1b（64.28%）,137 cases of type 2a（30.57%）,13 cases of type 1a（2.90%）,4cases of type 3b（0.90%）,2cases of type 3a（0.45%）,2cases of type 6a（0.45%）,2cases of type 1a1b（0.45%）.The analysis of HCV genotype and sex and age showed no significant correlation.The analysis of genotype prevalence in 2013-2016 years showed no obvious change trend.Conclusion Hepatitis C virus infection in Shandong area is dominated by type 1b,followed by type a2;there was no significant difference in age and gender,in the past 4years,there is no obvious change trend of genotype annual prevalence in Shandong area.
作者
孙雯雯
王秀芹
陈冬哲
司海霞
刘玲玲
SUN Wenwen WANG Xiuqin CHEN Dongzhe SI Haixia LIU Lingling(Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, China Shandong Jiaxiang Maternal and Child Health Care Center for Family Planning ,Zaozhuang, Shandong 272400,China)
出处
《检验医学与临床》
CAS
2017年第16期2338-2339,共2页
Laboratory Medicine and Clinic
基金
山东省临床重点专科建设项目经费资助项目(201326)
