摘要
目的探讨血清层粘连蛋白与透明质酸诊断肝纤维化的价值。方法选取2015年2月~2017年2月在廊坊市人民医院确诊为慢性乙型肝炎患者120例,其中轻度慢性肝炎患者35例,中度慢性肝炎30例,重度患者25例,肝硬化患者30例,选取同期30例健康体检者作为正常对照。收集患者和血清,层粘连蛋白采用磁微粒化学发光法检测,透明质酸用化学发光法检测,并对患者进行肝脏穿刺病理学检查,确定其纤维化程度,分析血清层粘连蛋白与透明质酸的水平与肝纤维化程度之间的关系。结果轻度肝炎患者的血清层粘连蛋白与正常对照差异无统计学意义(P>0.05)。中度患者、重度患者、肝硬化患者层粘连蛋白逐渐升高,在肝硬化患者中高达(190.77±38.65)ng/L,且均显著高于正常对照,差异有统计学意义(P<0.05)。血清透明质酸水平在所有慢性肝炎患者中的表达均显著高于正常对照,差异有统计学意义(P<0.05),轻、中、重度患者、肝硬化患者血清透明质酸水平呈上升趋势,差异有统计学意义(P<0.05)。肝硬化S_1、S_1、S_3、S_4期患者的层粘连蛋白和透明质酸均呈上升趋势,与S0期比较,差异有统计学意义(P<0.05),S_1、S_1、S_3、S_3期比较,差异有统计学意义(P<0.05)。结论血清层粘连蛋白及透明质酸可以反映肝组织纤维化程度,具有诊断价值。
Objective To investigate the diagnosis value of serum laminin and hyaluronic acid in hepatic fibrosis. Methods From February 2015 to February 2017, in People＇s Hospital of Langfang City, 120 patients diagnosed with chronic hepatitis B were selected, amond them, mild hepatitis patients were 35 cases, moderate hepatitis patients were 30 cases, serious hepatitis patients were 25 cases, liver cirrhosis were 30 eases, and 30 healthy physical examinees were selected as normal control at the same time. The serum of patients and physical examinees were collected, laminin was tested by magnetism particulate, hyaluronic acid was tested by chemiluminiscence, liver tissue of patients were punc- tured for pathology examination, degree of fibrosis was determined, the relationship between the serum laminin and byaluronic acid levels with the degree of liver fibrosis was analyzed. Results Laminin of mild hepatitis was compared with normal control, the difference was not statistically significant （P 〉 0.05）. Laminin of moderate, serious hepatitis and liver cirrhosis patients showed gradually rising, that of liver cirrhosis patients was the highest, it was （190.77＋_38.65） rig/L, and these were higher than that of normal control, the differences were statistically significant （P 〈 0.05）. Serum hyaluronic acid levels of chronic hepatitis patients were higher than that of normal contol, the difference was statistically signifi- cant （P 〈 0.05）, serum hyaluronic acid levels of mild, moderate, serious hepatitis and liver cirrhosis patients showed gradually rising, the differences were statistically significant （P 〈 0.05）. The serum hyaluronic acid levels of liver cirrhosis S1, S1, S3, S4 stage showed gradually rising, and these were compared with So stage, the differences were statistically significant （P 〈 0.05）. Conclusion Serum laminin and hyaluronic acid can reflect the degree of liver fibrosis, with diagnostic value.
出处
《中国医药导报》
CAS
2017年第23期111-113,121,共4页
China Medical Herald
基金
河北省廊坊市科技支撑计划项目(2015013028)
