摘要
目的建立高效液相色谱法(high performance liquid chromatography,HPLC)测定毛蕊异黄酮葡萄糖苷含量的分析方法,并对比不同产地中毛蕊异黄酮葡萄糖苷的含量。方法对不同产地黄芪样品采用甲醇加热回流提取,提取溶液采用反相色谱柱ZORBAX SB-C_(18)(4.6 mm×250 mm,5μm)检测,以乙腈:0.2%甲酸水梯度为流动相,流速1.0 m L/min,柱温30℃,检测波长260 nm。结果毛蕊异黄酮葡萄糖苷在0.2590~51.8000μg/m L范围内与峰面积积分值成良好的线性关系(r2=1.0000),毛蕊异黄酮葡萄糖苷平均加样回收率为106.5%,RSD为1.04%。不同黄芪由于产地和种植不同,毛蕊异黄酮葡萄糖苷含量差异较为明显。结论该方法简单、灵敏、稳定、可靠,可用于不同黄芪中毛蕊异黄酮葡萄糖苷的含量测定。
Objective To establish a method for determination of calycosin-7-glucoside in Astragalus membranaceus by high performance liquid chromatography（HPLC）, and compare the content of calycosin-7-glucoside in A. membranaceus from different origins. Methods A. membranaceus samples were extracted by heating reflux in methanol solution. Separations of extraction solution were carried out on a ZORBAX SB-C18 column（4.6 mm×250 mm, 5 μm）. The mobile phase was acetonitrile： 0.2% formic acid water gradient elution, the flow was 1.0 m L/min, the column temperature was 30 ℃, and the detection wavelength was 260 nm. Results Calycosin-7-glucoside had a good linearity with the peak areas at the range of 0.2590～51.8000 μg/m L（r^2=1.0000）. The average recovery was 106.5% and the RSD was 1.04%. The difference of calycosin-7-glucoside content was obvious in A. membranaceus from different origins and cultivations. Conclusion The established method is simple, sensitive, specific, and reproducible, which can be used to determine the content of calycosin-7-glucoside in A. membranaceus.
出处
《食品安全质量检测学报》
CAS
2017年第1期223-226,共4页
Journal of Food Safety and Quality
基金
上海市科委科研计划项目(14142201100)~~
关键词
黄芪
毛蕊异黄酮葡萄糖苷
高效液相色谱法
含量测定
Astragalus membranaceus
calycosin-7-glucoside
high performance liquid chromatography
content determination
