摘要
采用RAPD技术分析了4个地区(合作、碌曲、玛曲、阿孜)不同花色(黄、浅、白)11个钝裂银莲花居群的遗传结构。结果表明:钝裂银莲花居群遗传多样性较高,Shannon多样性指数(I)为0.6025,Nei’s基因多样性指数(H)为0.4166,Nei’s基因分化系数(Gst)和AMOVA分析表明钝裂银莲花居群间分化程度较低,大部分变异存在于居群内(67%),居群间占一小部分(33%)。对不同居群进行UPGMA聚类分析,结果显示11个亚居群可以归为3大支。相关性分析表明遗传多样性与地理分布和土壤营养状况有相关性。
The genetic structure of 11 different populations of floral-color Anemone obtusiloba in 4 different areas of Gannan were evaluated using random amplified polymorphism DNA（RAPD） .The results showed that the genetic diversity in Anemone obtusiloba populations was quite high ： The Shannon index （I） was 0.6025 and Nei＇ s gene diversity （H） was 0.4166. Moreover, the differentiation level among populations was low based on Nei＇ s Gst value and analysis of molecular variance （AMOVA）. Less than 33% of genetic diversity was attributed to the differentiation among populations while more than 67% resided within the different populations.According to unweighted pair group method using arithmetic averages （UPGMA）result, 11 populations of A.obtusiloba were clustered into 3 groups. Further correlation analysis showed that genetic diversity and distance correlated with geographical distribution and soil nutrition.
出处
《食品工业科技》
CAS
CSCD
北大核心
2017年第17期141-145,259,共6页
Science and Technology of Food Industry
基金
国家自然科学基金(30960066)
