摘要
目的体外诱导月经血子宫内膜间充质干细胞（MenSC）向子宫内膜上皮细胞分化。方法培养MenSC和子宫内膜间质细胞（ESC）并鉴定，将MenSC与ESC共培养，同时补充生长因子（TGF—β10ng／ml，EGF10ng／ml，PDGF—BB10ng／m1）和雌激素（17-β-雌二醇1×10^-7mol／L），之后免疫荧光染色检测分化的MenSC是否表达上皮细胞特异标记细胞角蛋白（CK）。结果MenSC生长良好，10代以内均表现出明显的梭状形态，结构紧密，其表面标记CD44、CD90、CD73和CD29表达率分别为98．4％、90．77％、94．75％和97．01％。ESC特异标记波形蛋白表达阳性。MenSC与ESC共培养3周后细胞形态基本由成纤维样向上皮样细胞转变，免疫荧光鉴定诱导分化后的MenSC表达细胞角蛋白。结论在ESC共培养环境的诱导下，MenSC可向子宫内膜上皮细胞分化。细胞生长因子和雌激素在这一进程中发挥重要作用。
Objective To study the potential of differentiation of menstrual blood-derived mesenchymal stem cells （MenSC） into endometrial epithelial cells （ EEC ） in vitro. Methods Endometrial stromal cells （ESC） and MenSC were cultured and identified by certain methods. MenSC were co-cultured with ESC, added 10 ng/ml TGF-β1, 10 ng/ml EGF, 10 ng/ml PDGF-BB and 1 × 10^-7 mol/L 17-β- estradiol. The expression of cytokeratin, which was marker of EEC, was tested by immunofluorescence in differentiated MenSC. Results MenSC proliferated quickly in vitro, and showed fusiform shape and clear structure within 10 generations. The expression rate of superficial markers of MenSC, CD44, CD90, CD73, and CD29, were 98.4% , 90. 77% , 94. 75% and 97.01% respectively. The expression of vimentin, which was stromal marker, was positive in cultured ESC （ P3 ）. After co-cultured with ESC for 3 weeks, MenSC morphology changed from fibroblast-like ceils into epithelial-like cells. Immunofluorescence assay showed that the differentiated MenSC expressed cytokeratin. Conclusion Under the condition of co-culture with ESC, MenSC has the ability to differentiate into endometrial epithelial cells. Cell growth factor and estrogen play the important roles in this process.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2017年第33期2614-2619,共6页
National Medical Journal of China
基金
国家自然科学基金（81270657
81471505）
浙江省自然科学基金（LYl3H040003）
浙江省重点研发计划项目（2017C03022）
