摘要
目的研究肾移植受者术后不同时间血清可溶性人类白细胞抗原G(s HLA-G)和可溶性CD30(s CD30)的水平变化,探讨血清s HLA-G和s CD30的表达与肾移植术后时间的关系。方法选取11例肾移植受者,并募集10例健康供者作为对照组,分别检测术前及肾移植术后1年内s HLA-G和s CD30水平的动态变化;选取33例移植肾功能正常受者,按照移植术后时间分为术后≤5年组、5年<术后时间≤10年组、术后>10年组,观察血清s HLA-G和s CD30在术后1年以上的水平。结果肾移植受者术前血清s HLA-G水平与对照组、术后1周、术后1月的水平相当;术后3月血清s HLA-G水平高于术后1月;术后3、6、12月三组之间s HLA-G水平无显著变化;术后>10年组显著高于术后≤5年组。血清s HLA-G水平与肾移植术后时间显著相关。术前s CD30水平高于正常对照组,术后1周较术前降低,术后1月较术后1周降低。术后1、3、6、12月之间及术后≤5年组、5年<术后时间≤10年组、术后>10年组之间s CD30水平无明显变化。s CD30水平仅与肾移植术后1月内时间显著相关。结论移植肾功能正常受者血清s HLA-G水平随着肾移植术后时间的延长而升高,而血清s CD30水平在肾移植术后1月内随时间延长而降低。
Objective To investigate the expressions of serum soluble human leukocyte antigen G（ s HLA-G） and soluble CD30（ s CD30） in renal transplant recipients at different time after transplantation,and explore the relationship between the expressions of serum s HLA-G,s CD30 and the time after renal transplantation. Methods Eleven kidney transplant recipients and 10 healthy donors were selected,in which the dynamic changes of serum s HLA-G and s CD30 were detected by ELISA before transplantation and 1 year after transplantation; 33 kidney transplant recipients with normal renal graft were selected and divided into three groups： 1-5 years,5-10 years and 10 years post-transplantation. The expressions of serum s HLA-G and s CD30 in the recipients were tested over one year after transplantation. Results The level of serum s HLA-G before transplantation was not significantly different from that of the control group. There was no significant difference between pre-transplantation,1 week and 1 month after transplantation. Serum s HLA-G level of renal transplant recipients at 3 months after transplantation was higher than that 1 month after transplantation. There was no significant change in serum s HLA-G level among 3,6 and 12 months after transplantation. The level of serum s HLA-G in the group of transplant time 10 years was significantly higher than that in the group of transplant time ≤5 years. The serum s HLA-G level was significantly associated with the time after renal transplantation. The level of serum s CD30 before transplantation was higher than that in the control group and decreased in 1 week after transplantation. There were no significant differences in s CD30 level between 1,3,6,and12 months after transplantation,and similarly,there were also no significant differences between the groups of transplant time≤5 years,5-10 years and 10 years after transplantation. The level of s CD30 was significantly associated with the time within 1month after renal transplantation. Conclusion The serum s HLA-G in kidney transplant recipients with normal renal graft increased with the time after renal transplantation,while the serum s CD30 level was reduced within 1 month after renal transplantation.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2017年第7期947-952,共6页
Chinese Journal of Cellular and Molecular Immunology
基金
高等学校博士学科点专项科研基金(20130201110059)
国家自然科学基金(81670681)
