摘要
目的探讨皮层肌动蛋白(Cortactin)在肺癌组织的表达与患者生存预后的关系及对肺癌细胞侵袭、迁移的影响。方法收集贵州省人民医院50例行肺叶切除术的非小细胞肺癌(NSCLC)患者癌组织及癌旁组织,免疫组化染色检测肺癌组织Cortactin表达,分析Cortactin表达与肺癌患者临床资料的关系。对肺癌患者进行随访,记录患者5年生存率;以5年生存率作为评价指标,采用单变量和多变量Cox比例风险模型评价患者预后的影响因素。采用小干扰RNA(siRNA)技术抑制肺癌A549细胞Cortactin的表达,划痕实验检测细胞迁移能力,Transwell实验检测细胞侵袭能力。结果肺癌组织Cortactin高表达率为72.0%(36/50),癌旁组织高表达率为24.0%(12/50),肺癌组织Cortactin高表达率明显高于癌旁组织,两组间比较差异具有统计学意义(P<0.01)。肿瘤最大径≥3cm组Cortactin高表达率明显高于肿瘤最大径<3cm组,中高分化组Cortactin高表达率明显高于低分化组,有淋巴结转移组Cortactin高表达率明显高于无淋巴结转移组(均P<0.05);Cortactin表达与性别、年龄、TNM分期、组织学类型无关(均P>0.05)。Cortactin低表达组5年总生存率为42.1%,高表达组5年总生存率为13.7%,高表达组5年总生存率明显低于低表达组(P=0.018)。Cox多因素分析显示肿瘤直径大、淋巴结转移、高Cortactin表达是影响患者总生存期的独立危险因素(均P<0.05)。划痕实验显示Cortactin-siRNA组细胞迁移率明显小于Cortactin-NC组、Cortactin-BC组;Transwell实验显示CortactinsiRNA组穿膜细胞数明显少于Cortactin-NC组、Cortactin-BC组,差异均具有统计学意义(均P<0.05)。结论 Cortactin表达与肺癌患者生存预后有关,抑制Cortactin表达能够降低肺癌细胞的侵袭、迁移能力,Cortactin有望成为肺癌治疗的新靶点。
Objective To explore the expression of cortactin in lung cancer tissues and its effects on invasion and migration.Methods Non-small cell lung cancer tissue samples were colleted from 50 patients in the People＇s Hospital of Guizhou Province.The expression of cortactin in tissues was mearsured by immunohistochemical staining.The relationship between cortactin expression and clinicopathological data was analysed.The patients with lung cancer were followed up postoperatively,and5-year survival was recorded.For 5-year survival as evaluation index,the prognostic factors were evaluated by univariate and multivariate Cox proportional hazards model.The expression of cortactin in lung cancer cell line A549 was inhibited by siRNA,the cell migration capability was mearsured by wound-healing assay,and the cell invasion capality was mearsured by transwelll assay.Results The expression rate of cortactin in cancer tissue was 72%（36/50）,and 24%（12/50）in paracancerous tissue,the expression rate of cortactin in cancer tissues was significantly higher than that in paracancerous tissues（P0.01）.The expression rate of cortactin in tumor was significantly higher in diameter≥3cm than that in tumor with diameter3cm,significantly higher in middle and high differentiated group than in poor differentiated group,significantly higher in lymph node metastasis group than in metastasis-free group（all P0.05）.Cortactin expression was not correlated with sex,age,TNM stage and histological type（P0.05）.The 5-year survival rate was 42.1%in low expression group,and 13.7%in high expression group;the5-year survival rate of high expression group was significantly lower than that of low expression group（P=0.018）.Cox multivariate analysis showed that tumor size,lymph node metastasis and high cortactin expression were independent risk factors for overall survival（all P0.05）.The result of wound-healing assay showed that migration cell number of cortactin-siRNA group was significantly less than that of cortactin-NC group and cortactin-BC group.Transwell assay showed that the number of transmembrane cells in cortactin-siRNA group was significantly less than that in cortactin-NC group and cortactin-BC group（all P0.05）.Conclusion The expression of cortactin is related to the prognosis of patients with lung cancer,inhibition of the expression of cortactin can reduce the invasion and migration capability of lung cancer cells,and cortactin is expected to be a new target for the treatment of lung cancer.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2017年第4期414-419,425,共7页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
贵州省2016年科技联合基金项目[No.黔科合作LH(2016)7182]
关键词
皮层肌动蛋白
肺癌
生存率
侵袭
迁移
cortactin
lung cancer
survival rate
invasion
migration
