摘要
目的 观察在结肠癌SW480细胞中Ca2＋浓度对钙周期素结合蛋白（CacyBP/SIP）的核转位及增殖、凋亡的影响。方法 采用免疫荧光染色观察不同浓度的钙离子载体（ionomycin）刺激结肠癌SW480细胞CacyBP/SIP的细胞内定位，噻唑蓝（MTT）法检测细胞存活率，流式细胞仪检测细胞周期与凋亡。结果 在结肠癌细胞SW480中，CacyBP/SIP在细胞质表达；1 μmol/L ionomycin刺激组CacyBP/SIP表达于细胞质；2 μmol/L刺激组CacyBP/SIP表达于胞质和胞核；5 μmol/L刺激组CacyBP/SIP表达于胞核；10 μmol/L刺激组CacyBP/SIP重新分布于胞质和胞核。不同浓度ionomycin组与未处理组比较，结肠癌SW480细胞的存活率无明显影响（（P＝172.918）。不同ionomycin浓度刺激组与对照组比较，对结肠癌SW480细胞的早期凋亡率[（0.747±0.049）%]和总凋亡率[（2.383±0.068）%]均无影响（P＝79.618）。结论 在结肠癌SW480细胞中，Ca2＋浓度可以影响CacyBP/SIP核转位，但不影响细胞的增殖和凋亡。
Objective To exploring the nuclear translocation of calcyclin（S100A6）-binding-protein （CacyBP）/SIP in response to elevated intracellular calcium concentration （[Ca2＋ ]i） by ionomycin in SW480 cells. And the effect of different [Ca2＋ ]i on proliferation and apoptosis in SW480 cells.Methods Immunofluorescence staining was used to detected the localization of CacyBP/SIP treated with inonmycin in SW480 cells. The cell survival rate was detected by methyl thiazol tetrazolium （MTT）. Cell cycle and apoptosis was detected by flow cytometry.Results Different concentration of ionomycin were treated with colon cancer SW480 cells and probed by immunocytochemistry staining using anti-CacyBP/SIP antibodies. CacyBP/SIP was distributed throughout the cytoplasm in untreated cells. In ionomycin-treated cells, CacyBP/SIP was also detected in the cytoplasm treated with 1 μmol/L and 2 μmol/L ionomycin. However, 5 μmol/L ionomycin caused a significant translocation of endogenous CacyBP/SIP from the cytoplasm to nucleus. While 10 μmol/L of ionomycin induces CacyBP/SIP retranslocation to cytoplasm and nucleus. The ionomycintreated cells showed no significant effect on cell proliferation by the MTT assay （P=172.918）. The results of the cell apoptosis analysis by FACS similarly indicate that ionomycin has no effect on the SW480 cells early apoptosis rate [（0.747±0.049）%] and total apoptosis [（2.383±0.068）%, P=79.618].ConclusionAlthough elevating [Ca2＋ ]i by ionomycin has affected on the nuclear translocation of CacyBP/SIP, it has no affected on the proliferation and apoptosis in SW480 cells.
出处
《中华实验外科杂志》
CSCD
北大核心
2017年第9期1513-1515,共3页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金（81072040）
宁夏自然科学基金（NZ0897）
宁夏医科大学重点资助项目（XZ201413）
关键词
结肠癌
钙离子
核转位
细胞增殖
凋亡
Colon cancer
Calcium
Nuclear translocation
Proliferation
Apoptosis
