摘要
目的 探讨白细胞介素（IL）-35在腹腔感染的脓毒血症患者中的表达水平及诊断价值。
方法 分别抽取50例腹腔感染所致脓毒血症患者、系统性炎性反应综合征（SIRS）与健康体检者外周血，检测血浆中白细胞计数（WBC）、C反应蛋白（CRP）、降钙素原（PCT）及IL-35表达水平，利用受试者工作特征（ROC）曲线分析这些指标的诊断价值。此外，评估腹腔感染脓毒血症组IL-35表达水平与急性生理功能和慢性健康状况评分系统Ⅱ（APACHE Ⅱ）评分相关性。结果 腹腔感染所致脓毒血症组IL-35表达水平显著高于SIRS组及健康对照组，差异有统计学意义（P＝0.000）。腹腔感染所致脓毒血症中死亡组IL-35表达明显高于存活组。ROC曲线结果显示，IL-35曲线下面积（0.86）显著大于PCT（0.78）、CRP（0.75）、WBC（0.70），能有效地区别诊断腹腔感染所致脓毒血症组与SIRS组。此外，腹腔感染所致脓毒血症组IL-35表达水平与APACHEⅡ评分呈正相关（r＝0.500，P＝0.043）。
结论 血浆IL-35可作为一个有效、敏感的指标快速诊断腹部感染所致的脓毒血症。
Objective The aim of the study was to investigate the level and diagnostic significance of interleukin （IL）-35 in acute abdominal conditions.
Methods Plasma specimens were obtained from 50 patients with abdominal sepsis, patients with systemic inflammatory response syndrome （SIRS） and healthy persons, respectively. Plasma IL-35, procalcitonin （PCT）, C-reactive protein （CRP）, white blood cells （WBC） were measured. The receiver operating characteristic （ROC） curve was used to compare the diagnostic values of IL-35, PCT, CRP and WBC. In addition, the correlation of plasma IL-35 and acute physiology and chronic health evaluation Ⅱ （APACHE Ⅱ） score in sepsis group was assessed by Spearman correlation analysis.
Results The plasma level of IL-35 in the sepsis group was significantly higher than the SIRS group and healthy group （both P=0.000）. The plasma level of IL-35 in the death group was markedly higher than the survival group. The ROC for the diagnosis of sepsis versus SIRS showed that the AUC of IL-35 （0.86） was greater than that of PCT （0.78）, CRP （0.75） and WBC （0.70）. Additionally, in the sepsis group, the plasma IL-35 level was positively correlated with APACHEⅡ score （r=0.500, P=0.043）.
Conclusion Plasma level of IL-35 might be a significantly sensitive and useful indicator for rapid diagnosis of abdominal sepsis.
出处
《中华实验外科杂志》
CSCD
北大核心
2017年第9期1562-1564,共3页
Chinese Journal of Experimental Surgery
