摘要
目的 探讨微小RNA（miRNA，miR）-126在结肠癌组织中的表达及其临床意义。
方法 采用反转录-聚合酶链反应（RT-PCR）检测miR-126在结肠癌组织的表达并分析其与临床病理特征关系。通过RT-PCR检测人结肠癌SW480细胞及正常结肠上皮细胞FHC中miR-126的表达水平；采用人工合成的miR-126模拟物瞬时转染人结肠癌SW480细胞，采用细胞计数试剂盒（CCK-8）分析转染后细胞增殖变化。
结果 RT-PCR结果显示，结肠癌组织miR-126阳性表达率为38.33%，显著低于癌旁组织（P＝0.017）；miR-126的低表达与患者的淋巴结转移（P＝0.005）、组织分化程度（P＝0.018）、肿瘤TNM分期（P＝0.000）明显相关，与患者的年龄、性别无明显相关（P＝0.350、0.819）。人结肠癌SW480细胞中miR-126的相对表达量为0.355±0.060，较FHC细胞显著降低，差异有统计学意义（P＝0.024）；采用人工合成的miR-126模拟物转染人结肠癌SW480细胞，SW480细胞中过表达miR-126可显著抑制细胞增殖（P＝0.012、0.003、0.000）。
结论 miR-126在结肠癌组织表达下调，影响结肠癌细胞的增殖，抑制结肠癌的发生发展。
Objective To discuss the expression of microRNA （miRNA, miR）-126 in colon cancer and its clinical significance.Methods The expression of miR-126 in colon cancer and cell line was detected by reverse transcriptase-polymerase chain reaction （RT-PCR） and its relationship with clinicopathological features was analyzed. We transfected the miR-126 mimics into SW480 cells, and cell counting kit-8 （CCK-8） was used to analyze cell proliferation.
Results The positive expression rate of miR-126 in colon cancer was 38.33%, which was significantly lower than that in adjacent normal tissues （P=0.017）. The low expression of miR-126 in colon cancer tissue was related to lymph node metastasis （P=0.005）, the degree of histological differentiation （P=0.018） and TNM staging （P=0.000）, but not to the age and sex of the patients （P=0.350, P=0.819）. The relative expression of miR-126 in colon cancer SW480 cells was 0.355±0.060, which was significantly lower than that of FHC cells （P=0.024）. Overexpression of miR-126 in SW480 cells could significantly suppress cell proliferation （P=0.012, 0.003, 0.000）.Conclusion The low expression of miR-126 in colon cancer can affect the proliferation of colon cancer cells and inhibit the development of colon cancer.
出处
《中华实验外科杂志》
CSCD
北大核心
2017年第9期1573-1575,共3页
Chinese Journal of Experimental Surgery
