摘要
目的:研究拳参乙酸乙酯萃取部位的化学成分,为其药效物质基础研究提供科学依据。方法:取拳参95%乙醇提取物浸膏的乙酸乙酯萃取部位,采用正相和反相柱色谱反复分离纯化,根据理化性质或波谱数据鉴定纯化后各化合物结构。结果:分离得到的11个单体化合物分别鉴定为β-谷甾醇(1)、二氢杨梅素(2)、槲皮素(3)、山柰酚(4)、表儿茶素(5)、绿原酸(6)、芦丁(7)、没食子酸(8)、1,2,3,4-四氢-8-羟基-4-异丙基-1-甲基萘-6-羧酸(9)、原儿茶酸(10)、鞣花酸(11)。结论:经文献查阅证实化合物2为首次从拳参植物中分离得到,化合物9为首次从蓼属植物中分离得到;本试验相关研究成果为进一步阐明拳参的药效物质基础提供了试验依据。
OBJECTIVE： To study the chemical constituents of the ethyl acetate extraction portion of Polygonum bistorta, pro- vide scientific basis for the basic research of its effective substances. METHODS： Taking the ethyl acetate extraction portion of P. bistorta 95 % ethanol extract, positive phase and reversed phase column chromatography were used for repeated isolation and purifi- cation, and the structures of the purified compounds were identified on the basis of physicochemical properties or spectral data. RE- SULTS： 11 monomer compounds were isolated and identified as fl-sitosterol （1）, dihydromyricetin （2）, quercetin （3）, kaempferol （4）, epicatechin （5）, chlorogenic acid （6）, rutin （7）, gallic acid （8）, 1,2,3,4-tetrahydro-8-hydroxy-4-isopropyl-l-methylnaph- thalene-6-carboxylic acid （9）, protocatechuic acid （10） and ellagic acid （11）. CONCLUSIONS： The literature is confirmed that compound 2 is isolated from P. bistorta for the first time and compound 9 is isolated from Polygonum for the first time, The related research results have provided experimental references for further clarifying the effective substances of P. bistorta.
出处
《中国药房》
CAS
北大核心
2017年第25期3494-3497,共4页
China Pharmacy
基金
河北省中医药管理局科研计划项目(No.2016064)
承德医学院自然科学研究计划(No.201621)
关键词
拳参
乙酸乙酯
萃取部位
化学成分
波谱数据
分离
鉴定
Polygonum bistorta
Ethyl acetate
Extraction portion
Chemical constituents
Spectral data
Isolation
Identifica- tion
