摘要
目的:对神香草40%乙醇洗脱物进行成分分析,并考察其对哮喘小鼠炎症的改善作用。方法:收集聚酰胺树脂柱40%乙醇洗脱物,采用高效液相色谱-高分辨率质谱技术对神香草40%乙醇洗脱物成分进行分析。将72只小鼠随机分为空白组(生理盐水)、模型组(生理盐水)、地塞米松组(阳性对照,1.6 mg/kg)和神香草40%乙醇洗脱物高、中、低剂量组(200、100、50 mg/kg),每组12只。除正常组外,其余各组小鼠均于实验第0、14天ip 0.2 m L卵白蛋白(OVA)致敏;于实验第25~31天ig给药,每天1次;给药结束后,用2 mg/mL OVA连续滴鼻激发7 d。末次滴鼻激发24 h后,检测各组小鼠肺泡灌洗液(BALF)中肿瘤坏死因子α(TNF-α)、白细胞介素4(IL-4)和干扰素(IFN-γ)水平;观察肺组织病理变化。结果:共鉴定出11种化合物,相对百分含量为40.89%,主要成分有迷迭香酸、木犀草素7-O-α-L-吡喃鼠李糖(1→6)-β-D-吡喃葡萄糖苷、金丝桃苷等。与空白组比较,模型组小鼠BALF中TNF-α、IL-4水平升高,IFN-γ水平降低,IL-4/IFN-γ比值增大(P<0.01);肺组织破坏严重,血管周围有炎症细胞浸润。与模型组比较,地塞米松组和神香草40%乙醇洗脱物高、中剂量组小鼠BALF中TNF-α、IL-4水平降低,IFN-γ水平升高,IL-4/IFN-γ比值减小(P<0.01);肺组织病理变化改善。结论:本研究建立的分析方法能够有效地分析神香草40%乙醇洗脱物中的化学成分;神香草40%乙醇洗脱物可调节哮喘小鼠肺组织炎症因子的释放,减轻哮喘小鼠肺组织的炎症病变。
OBJECTIVE： To conduct component analysis for the 40% ethanol eluate of Hyssopus officinalis, and investigate its improvement effect on inflammation in asthmatic mice. METHODS： The 40% ethanol eluate of polyamide resin column was col- lected, and HPLC-high resolution mass spectrometry was used for the component analysis of 40% ethanol eluate of H. officinalis. Totally 72 mice were randomly divided into blank group （normal saline）, model group （normal saline）, dexamethasone group （positive control, 1.6 mg/kg） and 40% ethanol eluate of H, officinalis high-dose, medium-dose, low-dose groups （200, 100, 50 mg/kg） , 12 in each group. Except for normal group, mice in other groups were intraperitoneally injected 0.2 mL ovalbumin （OVA） for sensitization in 0, 14 d and intragastrically administrated in 25-31 d, once a day. After administration, 2 mg/mL OVA was dropped in nose for 7 d. After 24 h of last dropping in nose, tumor necrosis factor α （TNF-α）, interleukin-4 （IL-4）, interfer- on-γ （IFN-γ） levels in bronchoalveolar lavage fluid （BALF） were detected; pathological changes in lung tissue were observed. RE- SULTS： Totally 11 compounds were identified, the relative percentage content of 40.89%. The main components were rosmarinic acid, luteolin 7-O-β-D-rhamnoserhamnose （1→6）-α-D-pyran glucoside, hyperoside, etc. Compared with blank group, TNF-α, IL-4 levels in BALF in model group were increased, IFN-γlevel was declined, and IL-4/IFN-γ ratio was enlarged （P〈0.01） ; lung tissue was seriously damaged, there was infiltration of inflammatory cells around the blood vessels. Compared with model group, TNF-α, IL-4 levels in BALF in dexamethasone group, 40% ethanol eluate of H. officinalis high-dose, medium-dose groups were declined, IFN-γ level was increased, and IL-4/IFN-γ ratio was reduced （P〈0.01） ; pathological changes in lung tissue were improved. CONCLUSIONS： The established analysis method can effectively analysis the chemical components of 40% etha- nol eluate of H. officinalis, which has certain regulatory effect on releasing inflammatory factors and reducing inflammatory lesions in lung tissue of mice with bronchial asthma.
出处
《中国药房》
CAS
北大核心
2017年第25期3532-3535,共4页
China Pharmacy
基金
国家自然科学基金资助项目(No.81560644)
新疆维吾尔自治区中医民族医药科技人才培养项目(No.2016-03-03)
关键词
维药
神香草
40%乙醇洗脱物
支气管哮喘
成分分析
炎症因子
小鼠
Wei medicine
Hyssopus officinalis
40% ethanol eluate
Bronchial asthma
Component analysis
Inflammatory factors
Mice
