摘要
目的:研究垂丝海棠内生真菌Penicillium oxalicum的代谢产物。方法:从垂丝海棠叶片中分离得到一株内生真菌CLP001,通过18S r DNA的ITS基因序列比对分析对其进行鉴定,并利用液体静置培养的方法得到发酵产物,以及利用硅胶,LH-20羟丙基葡聚糖凝胶,半制备HPLC等多种柱色谱技术对其分离纯化,并根据化合物的光谱数据和理化性质进行结构鉴定。结果:内生真菌CLP001与P.oxalicum的相似性达到99%,因此被鉴定为P.oxalicum CLP001,从内生真菌发酵产物中分离鉴定了10个化合物,分别为亚油酸甘油三酯(1),1,2-亚油酸甘油二酯(2),1-单亚油酸甘油酯(3),单乙酸甘油酯(4),棕榈酸(5),亚油酸(6),麦角甾醇(7),1-D-阿拉伯醇-单亚油酸酯(8),尿嘧啶(9),反-丁烯二酸(10)。结论:其中化合物1~4以及8,10为首次从该属中分离得到。
Objective： To study the metabolites from the endophytic fungus Penicillium oxalicum of Malus halliana. Method： An endophytic fungus CLP001 was separated from the leaves of M. halliana and identified through its 18SrDNA ITS sequence analysis. The fermentation products were obtained by liquid static culture method, and further isolated and purified by silica gel, Sephadex LH-20 column chromatograghy and preparative HPLC method, and their structures were identified through spectral data and physiochemical properties. Result： The endophytic fungus CLP001 was identified as P. oxalicum CLP001 on basis of their similarity of 99%. Ten compounds were isolated and identified as trilinolein （1）, 1, 2-dilinolein （2）, 1-monolinolein （3）, monacetin （4）, palmitic acid （5）, linoeic acid （6）, ergosterol （7）, 1-D-arabinitol-monolinoleate （8）, uracil （9）, and （E） -butenedioic acid （10）. Conclusion： Among them, compounds 1-4, 8 and 10 were isolated for the first time from this genus.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2017年第17期53-57,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(31501552)
郑州市科技局科技攻关项目(20150347)
关键词
内生真菌
垂丝海棠
草酸青霉
代谢产物
endophytic fungi
Malus halliana
Penicillium oxalicum
metabolites
