摘要
研究用盖玻片、滤纸和盖玻片+擦镜纸3种血清蛋白醋酸纤维薄膜电泳点样方法对电泳结果的影响,评价盖玻片与擦镜纸相结合的新型点样方法在教学实验中的应用效果,并分析3组的蛋白质相对含量有无差异。结果表明:在相同实验条件下,盖玻片+擦镜纸组的电泳结果优于其他2种,3组的蛋白质相对含量无明显差异;盖玻片与擦镜纸相结合的点样方法较单纯使用盖玻片或滤纸的点样方法点样成功率有很大提高,出现的不良结果较少,能有效解决学生在电泳实验操作中的点样问题,可在教学实验中推广。
The effect of the serum protein acetate membrane electrophoresis by the three spotting methods of the coverslip,filter paper and coverslip ＋ lens cleaning paper on the electrophoresis results is studied.The application effect of the new spotting method with the combination of the coverslip and the lens cleaning paper is evaluated and the difference of relative protein content of the three groups is analyzed.The results show that under the same experimental conditions,the electrophoresis result of coverslip＋lens cleaning paper group is better than that of the other 2 groups,and there is no significant difference in the protein relative content of the 3 groups.The spotting method with the combination of the coverslip with the lens cleaning paper is better than the spotting methods of the coverslip and the filter paper,which greatly improves the successful rate.It can effectively solve the students＆#39;spotting problem in the operation of electrophoresis experiments,and can be popularized in the teaching experiment.
出处
《实验技术与管理》
CAS
北大核心
2017年第8期60-62,83,共4页
Experimental Technology and Management
基金
河南省教育科学十二五规划课题(2014-JKGHC-0100)
国家级大学生创新创业训练计划项目(201610471013)
