摘要
目的建立不同产地皂角刺HPLC指纹图谱,为皂角刺质量评价提供科学依据。方法采用RP-HPLC法,色谱柱为Agilent ZORBAX SB-Aq C_(18)(250 mm×4.6 mm,5μm),流动相为甲醇(A)-体积分数为0.1%的乙酸溶液(B),梯度洗脱,检测波长为254 nm,柱温为35℃,流速为1.0 m L·min-1。采用中药色谱指纹图谱相似度评价系统(2012.130723版本)和SPSS 21.0软件对谱图分别进行相似度分析和聚类分析。结果建立了皂角刺HPLC指纹图谱,确定了29个共有峰,并指认了其中5个共有峰的化学成分。从HPLC指纹图谱结果看,不同产地皂角刺化学成分相似,但含量差异较大。结论中药皂角刺HPLC指纹图谱可为中药皂角刺质量评价提供科学依据。
Objective To establish the HPLC fingerprint of Gleditsiae spina from different regions for identification ,quality assessment and resource evaluation. Methods The samples were extracted with 70% （φ） ethanol aqueous and then analysis was carded out on an Agilent ZORBAX SB-Aq C18 column（250 mm ×4.6 mm,5μm） with the mobile phase consisting of methanol （A） and 0.1% （φ） acetic acid solution （ B ）. Gradient elution was carried out at the flow rate of 1.0 mLo min-1. The column temperature was set at 35 ℃, and the detection wavelength was 254 nm. The software ＂ Similarity Evaluation System for Chromatographic Fingerprint of TCMs （ Version 2012. 130723 ）＂ was employed to generate the mean chromatogram and carry out the similarity analysis of the samples. SPSS 21.0 was employed to carry out the cluster analysis. Results The HPLC fingerprint of Gleditsiae spina from different regions was established for identification and determination of 29 common peaks. Sample chemical composition from different regions was similar, but the content difference was obvious. Conclusions The HPLC fingerprint of Gleditsiae spina was set up for the first time and which provides reference for the identification and quality assessment of Gleditsiae spina.
出处
《沈阳药科大学学报》
CSCD
北大核心
2017年第8期660-666,共7页
Journal of Shenyang Pharmaceutical University
基金
"重大新药创制"科技重大专项课题(中药质量安全检测和风险控制技术平台
2014ZX09304307-002)
广东省食品药品检验检测技术创新专项资金项目(广东道地药材的药效物质基础和质量标准研究
GDMDT-2015-206)
