摘要
目的探讨类风湿性关节炎(RA)患者关节腔积液中淋巴毒素β受体(LTβR)信号通路相关分子的表达情况,了解LTβR信号通路与类风湿性关节炎之间的关联性。方法利用实时荧光定量PCR技术检测类风湿性关节炎患者(22例)和骨关节炎患者(25例)关节腔积液标本中LTβR mRNA、LTαmRNA、LTβmRNA、p65 mRNA、Rel B mRNA的表达情况,分析LTβR信号通路相关分子在RA和骨关节炎患者中表达差异。结果类风湿性关节炎组患者关节腔积液中LTβR mRNA和Rel B mRNA的表达高于骨关节炎组,差异均有统计学意义(Z值分别为-4.399、-2.167,P<0.05),而LTαmRNA、LTβmRNA及p65 mRNA相对含量在2组间差异无统计学意义(Z值分别为-1.595、-1.461、-1.493,P>0.05)。结论 LTβR mRNA、Rel B mRNA在类风湿性关节炎患者关节腔积液中高表达,在类风湿性关节炎患者诊断中具有一定的价值,提示其可能在类风湿性关节炎的发生、发展过程中发挥一定的作用,且LTβR信号通路可能以与配体结合后激活非经典NF-κB途径的方式参与作用。
Objective To investigate the expression of related molecules of lymphotoxin β receptor（ LTβR） signal pathway in join fluid of patients with rheumatoid arthritis（ RA）,to undretand the relevance of LTβR signal pathways with rheumatoid arthritis. Methods The real-time fluorescent quantitative PCR（ Q-RT-PCR） technology was used to detect LTβR mRNA,LTαmRNA,LTβ mRNA,p65 mRNA and Rel B mRNA expression in articular cavity effusion specimens with rheumatoid arthritis（ RA,22 cases） patients and osteoarthritis（ OA,25 cases） patients. The expression differences of LTβR signal pathway related molecules with RA and OA patients were analyzed. Results The LTβ mRNA and Rel B mRNA expression in RA group patient were higher than those in OA group. The difference was statistically significant（ Z values were-4. 399,-2. 167,respectively,P〈0. 05）. The expressions of LTα mRNA,LTβ mRNA and p65 mRNA in RA group were higher than those in OA group,but the difference was not statistically significant（ Z values were-1. 595,-1. 461,-1. 493,respectively,P〉0. 05）. Conclusion LTβR mRNA and Rel B mRNA were highly expressed in the articular cavity effusion of patients with rheumatoid arthritis,and they have certain value in diagnosis. It showed that it may play a role in the occurrence and development of rheumatoid arthritis and the LTβR signaling pathway may be involved in the activation of the NF-κB pathway after ligand binding.
出处
《中国卫生检验杂志》
CAS
2017年第15期2190-2192,共3页
Chinese Journal of Health Laboratory Technology
基金
浙江省医药卫生科技计划项目(2015KYB242)
温州市公益性科技计划项目(Y20140609)
