摘要
目的:为了了解桂郁金种质资源的遗传多样性和亲缘关系。方法:利用SSR分子标记技术对广西12个不同产地的35份桂郁金种质进行分析,并运用NTsys 2.10e软件进行聚类。结果:11条引物扩增得到片段52条,其中35条为多态性片段,多态率达67.31%,遗传相似系数的范围在0.63~1.00之间。结论:广西12个不同产地的桂郁金种质存在着较大的差异,遗传多样性较丰富。该结果可以为桂郁金优良种质资源筛选,良种繁育等研究提供一定的参考依据。
Objective： To understand Curcuma kwangsiensis genetic diversity and genetic relationship of germplasm resources. Methods： Use SSR molecular marker technology for analyzing 35 Curcuma kwangsiensis germplasm from 12 different producing areas in Guangxi and use NTsys 2. 10 e software to carry on the clustering. Results： Eleven primers amplification had 52 fragments,including 35 polymorphism fragments. The polymorphic rate was 67. 31% and the scope of the genetic similarity coefficient was between 0. 63 to 1. 00. Conclusion： Curcuma kwangsiensis from twevle different producing areas in Guangxi have big differences in germplasm and more abundant genetic diversity. The results can be as Curcuma kwangsiensis excellent germplasm screening,well-bred breeding research to provide certain reference basis.
出处
《中华中医药学刊》
CAS
北大核心
2017年第9期2296-2298,共3页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金项目(81160500)
广西科技攻关重大专项项目(桂科攻1099063-2)
广西研究生教育创新计划项目(YCSZ2015181)
关键词
桂郁金
种质
SSR
遗传多样性
Curcuma kwangsiensis
germplasm
SSR
genetic diversity
