摘要
透明质酸是由葡萄糖醛酸和N-乙酰葡萄糖胺组成的双糖单位聚合而成的直链酸性粘多糖,在医药、化妆品、食品等领域拥有庞大的市场。传统研究通过优化发酵参数改善透明质酸的生产虽然取得了显著成效,但也趋于上限,加之天然生产菌株固有的发酵培养基成本高、具有一定致病性等等的劣势也日益显著。随着分子生物学技术的迅速发展以及对透明质酸合成相关基因研究的不断深入,研究重点逐渐转向利用基因工程技术构建高产、安全、具有特定分子量的透明质酸工程菌株。以下就有关透明质酸生产菌株基因工程改造的策略及研究进展进行概述和展望。
Hyaluronic acid is a linear acid mucopolysaccharide composed of repeating disaccharide glucuronic acid and N-acetyl-glucosamine units,which is widely used in medicine,cosmetics,food and other fields. Traditional studies have made significant achievements in improving the production of hyaluronic acid by optimizing the fermentation parameters,but have reached the upper limit,and the natural strains have the increasing disadvantages of high cost of fermentation medium and pathogenicity. With the rapid development of molecular biology technology and the continuous research on the genes related to hyaluronic acid synthesis,the research focus has gradually shifted to the use of genetic engineering technology to construct high yield,safe and specific molecular weight hyaluronan genetically engineered strains. Here the strategies and research progress of genetic engineering for the production of hyaluronic acid were reviewed.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2017年第8期72-77,共6页
China Biotechnology
基金
国家"863"计划资助项目(2014AA022107)
关键词
透明质酸
多糖
基因工程
分子量
Hyaluronic acid
Polysaccharide
Genetic engineering
Molecular weight
