摘要
目的:研究结肠癌组织中RNF181、TPX2的表达量及其与癌细胞活力、血管新生的相关性。方法:收集在我院接受手术切除的结肠癌组织及癌旁组织,制作石蜡切片并对RNF181、TPX2进行免疫组化染色,判断蛋白阳性率;抽提RNA并进行荧光定量PCR反应,测定RNF181、TPX2、VEGF、VEGFR1、VEGFR2、HIF-1α的mRNA表达量;采集结肠癌患者的术前血清标本并测定CEA、CA199、CA242的含量。结果:结肠癌组织中RNF181的mRNA表达量及蛋白阳性率均显著低于癌旁组织,TPX2的mRNA表达量及蛋白阳性率均显著高于癌旁组织;结肠癌组织中RNF181阳性患者的血清CEA、CA199、CA242的含量以及结肠癌组织VEGF、VEGFR1、VEGFR2、HIF-1α的mRNA表达量均显著低于RNF181阴性患者;结肠癌组织中TPX2阳性患者的血清CEA、CA199、CA242的含量以及结肠癌组织VEGF、VEGFR1、VEGFR2、HIF-1α的mRNA表达量均显著高于TPX2阴性患者。结论:结肠癌组织中低表达的RNF181、高表达的TPX2与癌细胞增殖过程以及血管新生过程密切相关。
Objective：To study the RNF181 and TPX2expression in colon cancer tissue and their correlation with cancer cell viability and angiogenesis.Methods：Colon cancer tissue and tissue adjacent to carcinoma that were surgically removed in our hospital between July 2013 and May 2016 were collected and made into paraffin sections,immunohistochemical staining for RNF181 and TPX2was conducted,and the positive protein rate was determined;RNA was extracted for fluorescence quantitative PCR reaction,and the mRNA expression of RNF181,TPX2,VEGF,VEGFR1,VEGFR2 and HIF-1αwere determined;the preoperative serum samples of colon cancer patients were collected to determine the contents of CEA,CA199 and CA242levels.Results：mRNA expression and positive protein rate of RNF181 in colon cancer tissue were significantly lower than those in adjacent tissue while mRNA expression and positive protein rate of TPX2 were significantly higher than those in adjacent tissue;CEA,CA199 and CA242levels in serum as well as VEGF,VEGFR1,VEGFR2 and HIF-1αmRNA expression in colon cancer tissue of patients with positive RNF181 in colon cancer tissue were significantly lower than those of patients with negative RNF181;CEA,CA199 and CA242levels in serum as well as VEGF,VEGFR1,VEGFR2 and HIF-1αmRNA expression in colon cancer tissue of patients with positive TPX2 in colon cancer tissue were significantly higher than those of patients with negative TPX2.Conclusions：The low expression of RNF181 and high expression of TPX2 in colon cancer tissue are closely related to the cancer cell proliferation and angiogenesis process.
出处
《海南医学院学报》
CAS
2017年第14期1969-1971,1975,共4页
Journal of Hainan Medical University
基金
武汉市卫计委公共卫生科研项目(WG13D12)~~
