摘要
目的:探讨转录因子T-bet、GATA-3、Foxp3及STAT-6在支气管哮喘患者中的作用,研究三伏天灸治疗支气管哮喘的免疫调节机理。方法:将18例支气管哮喘患者纳入治疗组,选取18例健康志愿者纳入对照组,运用实时荧光定量PCR检测支气管哮喘患者治疗前、治疗6个月后和对照组样本外周血单个核细胞(PBMNC)中T-bet、GATA-3、Foxp3和相关信号通路STAT-6的mRNA表达,采用流式细胞术检测外周血Th1、Th2比例,酶联免疫吸附法(ELISA)检测IFN-γ、IL-4水平。结果:经三伏天灸治疗后,支气管哮喘患者IFN-γ分泌降低,T-bet、GATA-3表达水平升高,STAT-6水平降低;治疗前后Foxp3水平差异无统计学意义(P>0.05)。结论:三伏天灸可下调STAT-6,上调T-bet、GATA-3,从而抑制Th2过度分泌,调节Th1/Th2比例平衡,在哮喘病程中起到免疫调节作用。
Objective： To investigate the role of transcription factors of T-bet, GATA-3, Foxp3 and STAT-6 in bronchial asthma patients, and study the immunoregulation mechanism of medicinal vesiculation in treatment of bronchial asthma. Methods： 18 cases of bronchial asthma patients were included in treatment group and 18 cases of healthy volunteers in control group; for the PBNMC samples of bronchial asthma patients those before and 6 months after treatment and control group, real-time PCR was used to detect the mRNA expression of T-bet, GATA-3, Foxp3 and related signaling pathway STAT-6, flow cytometry to detect Th1/Th2 ratio and ELISA to detect IFN-γ, and IL-4 levels. Results： After medicinal vesiculation treatment, IFN-γ secretion in bronchial asthma patients had decreased, expression levels of T-bet and GATA-3 had increased and STAT-6 level decreased; there was no significant difference between Foxp3 level that before and after treatment （P〉0.05）. Conclusion： Medicinal vesiculation can cut down STAT-6 and raise T-bet and GATA-3, thus inhibiting excessive secretion of Th2 and regulating the balance of Th1/Th2 ratio, which plays an immunoregulation role in course of asthma.
出处
《按摩与康复医学》
2017年第17期29-32,共4页
Chinese Manipulation and Rehabilitation Medicine
基金
广东省中医药局课题:编号:20141076
