摘要
为研究佛手柑内酯对大鼠成骨细胞增殖、分化的影响。原代培养新生大鼠颅骨细胞,利用MTT法、微量酶标法、酶联免疫吸附法(ELISA)、q PCR等方法分别测定不同浓度佛手柑内酯对大鼠成骨细胞增殖,碱性磷酸酶(ALP),骨钙素(BGP),Ⅰ型胶原mRNA(CollagenⅠmRNA)表达的影响。结果显示,与对照组相比,佛手柑内酯作用于细胞24、48、72 h均对成骨细胞的增殖有促进作用(P<0.05);作用48、72 h均能促进ALP、BGP、CollagenⅠmRNA表达(P<0.05)。表明佛手柑内酯可促进大鼠成骨细胞的增殖和分化,为防治骨质疏松症的新药研究提供理论依据。
To study the effects of bergapten （5-MOP） on proliferation and differentiation of cultured osteoblasts in vitro, MTT,microtitration assay,enzyme-linked immunosorbent method （ELISA） and qPCR were used separately to observe the proliferation, the activity of ALP and the content of osteocalcin and mRNA expression of collagen I of osteoblasts cul-tured in vitro with bergapten （5-MOP） of different concentrations in it for different incubation periods. The effect of pro-moting proliferation of rat osteoblasts cells in vitro after 24 h and 48 h and 72 h was more marked by the groups of ber-gapten （5-MOP） compared with the control group. The activity of alkaline phosphate （ALP） and secretion of osteocalcin （BGP） and mRNA expression of collagen I were stimulated by bergapten （5-MOP） after 48 h and 72 h incubation. It was demonstrated that bergapten （5-MOP） had the effects on stimulating the proliferation and differentiation of cultured osteoblasts in vitro.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2017年第9期1563-1567,共5页
Natural Product Research and Development
基金
河北省自然科学基金资助项目(C2009001072)
河北省科学技术研究与发展计划项目(河北省科技支撑计划项目09276418D-14)
河北省中医药管理局课题(2007116)
关键词
成骨细胞
增殖
分化
佛手柑内酯
osteoblast
proliferation
differentiation
bergapten
