摘要
目的研究内毒素亲和吸附剂(EAA)对创伤失血性休克大鼠肠损害及炎症因子水平的影响。方法选取SD大鼠,按照体重随机分为5组:正常组、模型组和低中高3个剂量实验组,每组10只。通过创伤和放血构建创伤失血性休克大鼠模型。在建模前30 min,低中高3个剂量(EAA 5,10,15 m L+15,10,5m L 0.9%Na Cl)实验组分别于大鼠消化道内灌注。用HE染色法观察大鼠肠道绒毛形态变化以及肠黏膜损伤程度。复苏后4,8,16 h对大鼠的内毒素、一氧化氮(NO)、肿瘤坏死因子α(TNF-α)水平进行检测。结果与空白组0.03±0.01比较,模型组的肠黏膜损伤指数为5.41±2.56,差异有统计学意义(P<0.05)。复苏4 h后,与模型组比较,高、中、低3个剂量实验组的大鼠肠黏膜损伤指数分别为3.45±0.75,2.53±0.68,2.02±0.53,差异均有统计学意义(均P<0.05)。与空白组比较,模型组大鼠的血清内毒素、NO、TNF-α水平分别为(456.52±21.56)pg·m L^(-1),(108.41±9.56)ng·m L^(-1),(77.58±10.65)ng·m L^(-1),差异均有统计学意义(均P<0.05)。复苏4 h后,高、中、低3个剂量实验组的大鼠内毒素、NO、TNF-α的水平分别为(280.41±15.42),(254.63±17.56),(221.53±13.53)pg·m L^(-1);(90.43±8.42),(63.54±7.53),(49.52±8.41)ng·m L^(-1);(77.58±10.65),(62.58±5.12),(42.51±4.53)ng·m L^(-1)。与模型组比较,3个剂量实验组的大鼠内毒素、NO水平差异均有统计学意义(均P<0.05);而TNF-α水平只有中低2个剂量实验组差异均有统计学意义(均P<0.05)。结论低浓度EAA能够有效缓解失血性休克引起的应激反应,降低炎症因子水平,从而保护大鼠肠功能。
Objective To investigate the effect of endotoxin affinity ad- sorbent （EAA） on intestinal injury and inflammatory factors in rats with traumatic hemorrhagic shock. Methods A total of 50 SD rats were ran- domly divided into five groups：normal group, model group, experimental low - close （ experimental - L group） , middle - dose （ experimental - M group） and high- dose （experimental- H group） groups, 10 rats in each group. Rats used for traumatic hemorrhagic shock model establi- shing were treated by making trauma and bloodletting. Before 30 min with model establishing, rats in the experimental- L, -M and -H groups were given （ EAA 5,10,15 mL ± 15,10,5 mL 0. 9% NaC1） respec- tively. The morphological changes of intestinal villi and intestinal mucosadamage in rats of those different groups were observed by HE staining. Resuscitation after shock for 4, 8 and 16 h, the changes of inflammatory factor levels, such as endotoxin, nitric oxide （NO） and tumor necrosis factor a （ TNF - a ）, in different groups were observed. Results Compared with blank group （0. 03 ± 0. 01 ）, the intestinal mucosal injury in- dex in model group was 5.41 ± 2.56 with significantly （P 〈 0. 05 ）. Resuscitation after shock for 4 h, compared with model group, the intestinal mucosal injury index in experimental - H, experimental - M, experimental - L groups were 3.45 ±0.75,2. 53 ±0. 68,2. 02 ±0. 53 with significantly（ all P 〈0. 05 ）. Compared with blank group, the endotoxin, NO and TNF - a in model group were （456.52 ± 21.56） pg ·mL-1, （ 108.41 ± 9. 56） ng · mL-l, （77.58 ± 10. 65 ） ng · mL-1 with significantly （ all P 〈 0. 05 ）. Resuscitation after shock tbr 4 h, the endotoxin, NO and TNF - a in experimental - H, experimental - M, experimental - L groups were （ 280. 41 ± 15.42 ）, （ 254.63 ± 17.56 ）, （221.53 ± 13.53）pg · mL-1; （90.43 ± 8.42）, （63.54 ±7.53）, （49.52 ± 8.41） ng · mL-1 ; （77.58 ±10.65）, （62.58 ±5.12）, （42. 51 ±4.53）ng · mL-1, respectively. Compared with model group, the endotoxin, NO in thee experimental groups had significantly （all P 〈 0. 05 ）while TNF -a in experimental -M, experimental -L groups had significantly （ all P 〈 0. 05）. Conclusion low - dose EAA could effectively relieve the stress response caused by hemorrhagic shock and reduce the level of inflammatory factors, thus it could protect the intestinal function of rats.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2017年第17期1675-1678,共4页
The Chinese Journal of Clinical Pharmacology
基金
河北省医学科学研究重点课题计划基金资助项目(ZD20140064)
关键词
内毒素亲和吸附剂
失血性休克
肠损伤
炎症因子
endotoxin affinity adsorbent
hemorrhagic shock
intestinal injury
inflammatory factor
