摘要
为了研究致病性粪肠球菌(Enterococcus faecalis)对脊尾白虾(Exopalaemon carinicauda)免疫相关基因表达的影响,分别对健康脊尾白虾注射生理盐水和粪肠球菌,测定了不同时间点血细胞和肝胰腺中酚氧化酶原(proPO)、C型凝集素(CTL)、超氧化物歧化酶(SOD)、组织蛋白酶D(Cat D)和组织蛋白酶L(Cat L)基因的相对表达量变化。结果显示,与对照组相比,感染粪肠球菌后,血细胞和肝胰腺中pro PO基因表达量均于6 h达到最大值(P<0.05),随后逐渐降低至对照组水平;血细胞和肝胰腺中CTL基因表达量分别于12 h和6 h上升至最大值,随后逐渐降低;血细胞中CTL基因表达量于72 h虽有降低,但仍显著高于对照组(P<0.05);血细胞和肝胰腺中SOD基因表达量分别于6 h和3 h达到最大值,随后逐渐降低,并于72 h达到正常水平;血细胞和肝胰腺中Cat D基因表达量变化趋势基本一致,呈现先降低后升高、然后显著降低再升高的趋势;血细胞和肝胰腺中Cat L基因表达量分别于6 h和3 h显著降低,随后逐渐升高至最大值,并于72 h达到对照组水平。研究表明,长时间的粪肠球菌感染对脊尾白虾免疫相关基因表达影响显著,本研究所用免疫相关基因均可作为粪肠球菌感染疾病的监测指标。
In this study, we investigated the effect of pathogenic Enterococcusfaecalis challenge on the non-specific immunity-related gene expression of Exopalaemon carinicauda. The expression levels of prophenoloxidase （proPO）, C-type lectin （CTL）, superoxide dismutase （SOD）, cathepsin D （Cat D） and cathepsin L （Cat L） in hemocytes and hepatopancreas were measured with quantitative real-time PCR at different sampling time points after healthy E. carinicauda were injected with 0.86% sterile saline （control） or pathogenic E. faecalis. Compared to the control group, after infected with E. faecalis, the relative expression levels ofproPO gene in hemocytes and hepatopancreas both increased to the highest at 6 h （P〈0.05）, and then decreased gradually back to normal. The relative expression levels of CTL gene in hemocytes and hepatopancreas increased to the peak value at 12 h and 6 h respectively （P〈0.05）, and then decreased gradually. Although the relative expression levels of CTL gene in hemocytes decreased at 72 h, it was still significantly higher than the control （P〈0.05）. The relative expression levels of SOD gene in hemocytes and hepatopancreas both increased to the highest at 6 h and 3 h respectively （P〈0.05）, then decreased gradually back to normal at 72 h. The dynamic expression of Cat D gene in hemocytes and hepatopancreas was consistent, which was a significant decreased in the beginning followed by an increase. After the decrease of expression at 12 h, it was increased significantly again. The Cat L gene expression in hemocytes and hepatopancreas both decreased significantly at 6 h and 3 h respectively （P〈0.05）, then it increased to the highest and recovered to the control level at 72 h. The results indicated that pathogenic E. faecalis challenge affected the immune status of E. carinicauda probably by changing the immunity-related gene expression, and that proPO, CTL, SOD, Cat D and Cat L were all sensitive to E. faecalis infection.
出处
《渔业科学进展》
CSCD
北大核心
2017年第5期148-155,共8页
Progress in Fishery Sciences
基金
国家虾产业技术体系(CARS-47)
国家自然科学基金(31472275)
山东省泰山产业领军人才工程项目(LNJY2015002)
青岛市产业培育计划科技惠民专项(17-3-3-62-nsh)
宁波市科技计划项目(2016C11011)
中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金(2016TS07)共同资助~~
