摘要
旨在对比分析Brevibacillus laterosporus BL-21和Bacillus subtilis HNDF2纯培养液和共培养液的差异,为研究两者的生态学关系和发现新抗菌物质提供参考。采用杯碟法检测抗菌活性,采用薄层层析、SDS-PAGE电泳以及HPLC分析代谢产物的差异。结果表明,共培养液对尖孢镰刀菌(Fusarium oxysporum)抗菌活性最高时的抑菌圈直径达到(28.50±0.5)mm,与B.laterosporus BL-21和B.subtilis HNDF2纯培养液的最高抑菌活性相比,分别提高了18.75%和83.90%。代谢产物检测结果表明,共培养液与B.laterosporus BL-21纯培养液的相似度较高,与B.subtilis HNDF2纯培养液的差别较大,并且含有各纯培养液中没有的新物质。2株菌的纯培养液和共培养液在抗菌活性和组分上存在显著差异,有必要对共培养液中的抗菌物质进行深入分析。
The aim of the study is to compare and analyze the differences between pure culture and co-culture metabolites of Brevibacillus laterosporus BL-21 and Bacillus subtilis HNDF2,and lay a foundation for further research on ecological relationship between the two strains and find new antibacterial substances from coculture broth.The antibacterial activity was detected by cylinder plate method.The differences of metabolites were analyzed by thin layer chromatography,SDS-PAGE and HPLC.The results showed that the maximum bacteriostatic diameter of co-culture broth against Fusarium oxysporum could reach（28.50±0.5）mm,compared with that of pure culture broth of B.laterosporus BL-21 and B.subtilis HNDF2,the antagonistic activity increased by 18.75%and 83.90%,respectively.The results of metabolites determination showed that the co-culture broth had a high similarity to the pure culture broth of B.laterosporus BL-21,but it had significant differences to the pure culture broth of B.subtilis HNDF2,and some new substances were detected in co-culture broth which did not exist in each pure culture broth.There were significant differences in antifungal activity and components of metabolites between pure culture broth and co-culture broth of the two strains,and it was necessary to further analyze the antimicrobial substances in co-culture broth.
出处
《中国农学通报》
2017年第22期118-125,共8页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金"侧孢短芽孢杆菌与枯草芽孢杆菌共培养抗菌活性增强的生态学机理"(31370511)
黑龙江省自然科学基金"侧孢芽孢杆菌与枯草芽孢杆菌共培养代谢产物的研究"(C201203)
黑龙江大学微生物学重点实验室开放项目"侧孢短芽孢杆菌与枯草芽孢杆菌共培养的生态学关系"(2012MOI-1)
关键词
侧孢短芽孢杆菌
枯草芽孢杆菌
共培养
抗菌代谢产物
生物防治
Brevibacillus laterosporus
Bacillus subtilis
co-culture
antibacterial metabolites
biological control
