摘要
建立了用高效液相色谱法同时测定白灵菇中3种荧光增白剂(VBL、CBS、CXT)的方法。试样用乙醇-水(V/V=1/2)溶液作提取剂,50℃条件下水浴静置提取15min。采用C18(250mm×4.6mm,2.6μm)为分析柱,以乙酸铵(20mmol/L)+乙腈[65+35]为流动相洗脱,流速为0.8mL/min。3种荧光增白剂在0.01~10μg/mL范围内线性关系良好,相关系数(r2)均大于0.997;加标回收率为79%~90%。方法简单准确,线性关系、重现性及加标回收率均满足分析要求。
A method for the determination of three fluorescent whitening agents （FWAs） （VBL, CBS, CXT） in mushroom by high performance liquid chromatography was developed. The sample was extracted with ethanol-water （V/V= 1/2） solution by static extraction of water for 15 min at 50 ℃. The HPLC method was performed on a column of Thermo Fisher Accueore C18 （250 mm× 4.6 mm, 2.6 μm） with elution using 20 mmol/L ammonium acetate （65%） and acetonitrile （35%） as the mobile phases with the flow rate of 0.8 mL/min. The target analytes showed good linearity in the range of 0. 01--10 μg/mL with the correlation coefficients （r2） greater than 0. 997. The recoveries were in the range between 79% -90%.
出处
《中国无机分析化学》
CAS
2017年第3期4-7,共4页
Chinese Journal of Inorganic Analytical Chemistry
基金
河北省高层次人才资助项目(A2016002037)资助
关键词
白灵菇
荧光增白剂
高效液相色谱
mushroom
fluorescent whitening agents（FWAs ）
high performance liquid chromatography（HPLC）
