摘要
食物过敏是由于接触特定过敏原引起的异常免疫反应。西红柿营养价值丰富并且可作为天然抗氧化剂,已被人们熟识并广泛食用的蔬菜。为了对西红柿中的内源性致敏蛋白有全面的认识和了解,本工作利用全序列比对的方法比较了西红柿蛋白与已知致敏原之间的序列相似性,获得了西红柿潜在致敏蛋白序列,并分析及描述了它们的保守结构域、分子作用功能、系统发育情况以及在染色体的位置。结果表明:共鉴定120个西红柿内源性致敏蛋白,其中包括9个已被数据库收录的西红柿致敏原;保守结构域分析显示它们分别属于43个蛋白家族,主要分布于Gelsolin、Chloroa_b-bind和Thaumatin等家族,其中的HATPase_c结构域未在已知致敏蛋白中发现;Chloroa_b-bind和Thaumatin家族蛋白在绿色植物较特有,将这2个家族蛋白进行系统发育分析则分别揭示了西红柿的遗传多样性;而且预测的致敏蛋白在西红柿染色体上有成簇状分布的趋势。
Food allergy is abnormal immune response caused by the contact with specific allergens. Tomatoes have rich nutrients and can be used as a natural antioxidant, which have been familiar by people and widely consumed. In order to have a clear understanding of endogenous allergens protein in tomatoes, full-length sequence alignment was performed to compare the similarities between tomato proteins and known allergens, In genome-wide, 120 potential allergens in tomato were identified and characterized by analyzing the conserved domain, molecular function, phylogenetic relation and distribution among the chromosomes. Among those tomato allergens, 9 proteins were known and listed in allergens databases. Conservative domain analysis revealed that they belonged to 43 protein families, respectively which mainly distributed in Gelsolin, Chloroa_b-bind and Thaumatin family. including one novel domain HATPase_c added to the allergen protein database. Phylogenetic analysis revealed the diversities among the Chloroa_b-bind and Thaumatin protein family, respectively. Additionally, some potential allergenic proteins clustered on the tomato chromosomes might suggest the molecular function during the evolution.
出处
《分子植物育种》
CAS
CSCD
北大核心
2017年第8期2963-2976,共14页
Molecular Plant Breeding
基金
2014年公益性行业科研专项(No.201403075)
2016转基因生物新品种培育重大专项课题(No.2016ZX08-012005-007)共同资助
