摘要
目的:建立HPLC同时测定地黄中毛蕊花糖苷及异毛蕊花糖苷含量的方法,比较鲜地黄及其炮制品中异毛蕊花糖苷的含量变化情况。方法:采用Waters-Symmetry ShieldTMRP18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.1%乙酸(18∶82),流速1 m L·min-1,检测波长334 nm,柱温30℃,进样量20μL。结果:在鲜地黄中暂未检测到异毛蕊花糖苷,而鲜地黄通过一定温度及时间的加工炮制后可产生异毛蕊花糖苷。清蒸不同时间的地黄样品中,毛蕊花糖苷及异毛蕊花糖苷含量变化属于一个动态变化过程,且蒸制时间对地黄中异毛蕊花糖苷含量影响较大;鲜地黄在一定温度下烘制可产生异毛蕊花糖苷成分,但烘制不同时间段,其含量变化不明显。毛蕊花糖苷与异毛蕊花糖苷在一定条件下可以相互转化。结论:地黄中异毛蕊花糖苷的产生部分由毛蕊花糖苷转化而来,可考虑将异毛蕊花糖苷也列为熟地黄的质量控制指标性成分之一。
Objective： To establish a method for determination of verbascoside and isoacteoside in Rehmanniae Radix simultaneously by HPLC,and to compare content changes of isoacteoside in Rehmanniae Radix and its processed products. Method： Contents of verbascoside and isoacteoside were determined by HPLC,Waters-Symmetry ShieldTMRP18 column（ 4. 6 mm × 250 mm,5 μm） was employed with a mobile phase of acetonitrile-0. 1% acetic acid solution（ 18 ∶ 82）, flow rate of 1 m L·min-1,column temperature at 30 ℃,detection wavelength of 334 nm and injection volume of 20 μL. Result： There was no isoacteoside in fresh Rehmanniae Radix temporarily, but through a certain temperature and time of processing, isoacteoside can be determined. In Rehmanniae Radix samples steamed at different times,the content change of verbascoside and isoacteoside was a dynamic change process,and the steaming time had a great effect on the content of isoacteoside in Rehmanniae Radix. Baked at a certain temperature,it can produce isoacteoside in fresh Rehmanniae Radix,but in the different time periods, the content of isoacteoside did not change obviously. Verbascoside and isoacteoside could be transformed into each other under certain conditions. Conclusion： Production of isoacteoside in Rehmanniae Radix is partly from the transformation of verbascoside; isoacteoside can be considered as a one of the quality control indexes of Rehmanniae Radix Praeparata.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2017年第18期22-26,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家公益性科研专项(201507002)
呼吸疾病诊疗与新药研发河南省协同创新中心项目(教科计[2013]638号)
关键词
地黄
熟地黄
炮制
毛蕊花糖苷
异毛蕊花糖苷
温度
烘制时间
Rehmanniae Radix
Rehmanniae Radix Praeparata
processing
verbascoside
isoacteoside
temperature
baking time
