摘要
目的:建立防风配方颗粒的HPLC含量测定方法,为防风配方颗粒的质量评价提供依据。方法:采用高效液相色谱法测定配方颗粒中升麻苷、升麻素、5-O-甲基维斯阿米醇苷、亥茅酚苷的含量,色谱柱为X-Bridge C18,流动相为乙腈-0.3%磷酸水溶液梯度洗脱,流速为1.0 mL/min,检测波长为220 nm,进样量为10μL。结果:升麻素、5-O-甲基维斯阿米醇苷、亥茅酚苷,升麻苷检测进样量线性范围为分别为0.21~4.12μg/mL(R=0.998 5),0.83~16.52μg/mL(R=0.998 3),0.31~6.20μg/mL(R=0.998 7),0.24~4.76μg/mL(R=0.998 2);精密度、稳定性、重复性试验的RSD<5%;加样回收率分别为97.82%(RSD=2.44%),95.18%(RSD=1.88%),99.33%(RSD=3.70%),95.45%(RSD=4.30%)。结论:HPLC法操作简便、稳定、重复性好,可用于防风配方颗粒中有效成分含量的测定。
Objective ：To establish the method for determinating 4 ingredients in formula granules of Saposhnik- oviae radix by HPLC, in order to provide the basis for quality evaluation of formula granules of Saposhnikoviae ra- dix. Methods ： The contents of prim - O - glucosylcimifugin, cimifugin, 4＂- 0 - 15 - D - glucosyl - 5 - O - methyl- visarnmioside and see - o - glucosylhamaudol in formula granules were determinated by HPLC. The chromatograph- ic column was X -Bridge C18; the mobile phase was aeetonitrile -0.3% phosphoric acid solution for gradient elution;the flow rate was 1 mL/min; the detection wavelength was 220 nm; and injection volume was 10 μL. Results：The linear ranges were respectively 0.21 -4.12 μg/mL（ R = 0.998 5 ） for cimicifugin,0.83 - 16.52 μg/ mL（ R = 0.998 3 ） for prim - O - glucosylcimifugin, 0.31 - 6.20 μg/mL（ R = 0.998 7 ） for 4＇- O - 15 - D - glu- cosyl - 5 - O - methylvisamminol, and 0.24 - 4.76 μg/mL （ R = 0.998 2） for sec - O - glucosylhamaudol. RSDs of precision, stability and reproducibility tests were lower than 5 %. The recovery rates of injection were respectively 97.82% （RSD = 2.44%, n = 5 ） for cimicifugin, 95.18 % （RSD = 1.88 %, n = 5 ） for prim - O - glucosyl- cimifugin, 99.33 % （ RSD = 3.70% , n = 5 ） for 4＂- O - 15 - D - glucosyl - 5 - 0 - methylvisamminol, and 95.45 % （ RSD = 4.30%, n = 5 ） for see - O - glucosylhamaudol. Conclusion ： This method is simple, stable and better reproducible, and can be used for simultaneous determination of contents of active ingredients in Saposhnikoviae ra- dix and its formula granules.
作者
李媛媛
倪艳
郝旭亮
LI Yuan- yuan NI Yan HAO Xu -liang(Shanxi Institute of Traditional Chinese Medicine, Taiyuan, 030012, China)
出处
《山西中医》
2017年第9期52-54,共3页
Shanxi Journal of Traditional Chinese Medicine
基金
山西省中医药研究院院级研究基金"玉屏风散药效物质基础研究"(编号:201412)
关键词
防风
配方颗粒
高效液相色谱法
质量评价
Saposhnikoviae radix, formula granules, HPLC, quality evaluation
