摘要
研究了过渡金属铜修饰的仲钨酸盐化合物[Na_2(H_2O)_(10)][Cu_4(H_2O)_(12)(H_2W_(12)O_(42))]·15H_2O(CuW_(12))的体外抗肿瘤活性.取人卵巢癌细胞SKOV-3加入不同浓度CuW_(12)处理,应用四甲基偶氮唑盐(MTT)比色法分析细胞增殖,计算了半数抑制浓度(IC_(50));采用光学显微镜观察SKOV-3细胞的凋亡形态变化;分析了细胞周期和细胞凋亡,计算了各期细胞比例及细胞凋亡率.结果表明:CuW_(12)对SKOV-3细胞增殖抑制的IC_(50)值为45.2μmol/L,且呈剂量依赖性;与经典的仲钨酸盐Na_(10)(H_2W_(12)O_(42))·26H_2O(NaW_(12))相比,过渡金属铜修饰的仲钨酸盐抗肿瘤活性更强;光学显微镜下观察到CuW_(12)处理组细胞数目明显减少,出现变圆、缩小、老化等形态变化;不同浓度CuW_(12)处理12h后早期凋亡细胞所占的百分比显著增加且呈剂量依赖性.
This paper has studied the in vitro anti-tumor activity of a novel paradodecatungstate-B compound decorated by transition metal copper：[Na2（H_2O）_（10）][Cu_4（H_2O）_（12）（H2W_（12）O_（42））]·15H_2O（abbreviated form：CuW_（12））.Human ovarian carcinoma SKOV-3 cell was treated with different concentration of CuW_（12）,then its IC_（50） was measured by MTT assay.The morphological change in SKOV-3 cell was observed by optical microscopes.Flow cytometry was applied to analyze cell cycle and apoptosis by computing the ratio of SKOV-3 cells in different phase and its apoptotic ratio.The results show that CuW_（12） can inhibit SKOV-3cell proliferation with IC_（50） of 45.2μ mol/L,showing dosedependent.Compared with CuW_（12） and classic paradodecatungstate compound Na_（10）（H2W_（12）O_（42））·26H_2O（abbreviated form：NaW_（12））,CuW_（12） exhibits higher inhibitory effect owing to the introduction of copper atom.Under the optical microscopes,we observed the number of SKOV-3 cells treated with CuW_（12） decreased significantly,cell rounding,shrinking and aging.Treated with different concentrations of CuW_（12） after 12 h,the proportions of SKOV-3 cells on the early apoptosis increased markedly,showing dose-dependent.
出处
《东北师大学报（自然科学版）》
CAS
CSCD
北大核心
2017年第3期92-96,共5页
Journal of Northeast Normal University(Natural Science Edition)
基金
国家自然科学基金资助项目(21301041)
吉林省教育厅科学技术研究项目(2015431)
关键词
仲钨酸盐
人卵巢癌细胞SKOV-3
抗肿瘤
铜
paradodecatungstate-B
human ovarian carcinoma SKOV-3 cells
anti-tumor
copper
