摘要
目的:建立同时测定板柴口服液中(R,S)-告依春和绿原酸含量的方法。方法:采用双波长高效液相色谱法。色谱柱为Wondasil C18,流动相为乙腈-0.1%磷酸溶液(11∶89,V/V),流速为1.0 m L/min,检测波长为245 nm[(R,S)-告依春]、327 nm(绿原酸),柱温为30℃,进样量为10μL。结果:(R,S)-告依春和绿原酸检测质量浓度线性范围分别为4.05~40.51μg/m L(r=0.9999)、29.41~294.05μg/m L(r=0.999 9);定量限分别为3.32、2.45 ng,检测限分别为1.00、0.74 ng;精密度、稳定性、重复性试验的RSD均<1.0%;加样回收率分别为98.46%~101.06%(RSD=0.98%,n=9)、98.18%~100.78%(RSD=0.86%,n=9)。结论:该方法操作简便,结果准确、灵敏度高、重复性好,可用于板柴口服液中(R,S)-告依春和绿原酸含量的同时测定。
OBJECTIVE： To establish a method for simultaneous determination of （R, S）-goitrin and chlorogenic acid in Ban- chai oral liquid. METHODS： The dual-wavelength HPLC method was adopted. The determination was performed on Wondasil C18 column with mobile phase consisted of acetonitrile-0.1% phosphoric acid （11 ： 89, V/V） at the flow rate of 1.0 mL/min. The detec- tion wavelengths were 245 nm for （R,S）-goitrin and 327 nm for chlorogenic acid. The column temperature was 30℃, and injec- tion volume was 10 μL. RESULTS： The linear ranges were 4.05-40.51 μg/mL for （R,S）-goitrin （r=0.999 9）, 29.41-294.05 μg/mL for chlorogenic acid （r=0.999 9）. The limits of quantification were 3.32, 2.45 ng, limits of detection were 1.00, 0.74 ng. RSDs of precision, stability and reproducibility tests were lower than 1.0% ; the recoveries were 98.46% -101.06% （RSD=0.98%, n=9） and 98.18%-100.78% （RSD=0.86% ,n=9）. CONCLUSIONS： The method is simple, accurate, sensitive and reproducible, and can be used for simultaneous determination of （R, S）-goitrin and chlorogenic acid in Banchai oral liquid.
出处
《中国药房》
CAS
北大核心
2017年第27期3853-3855,共3页
China Pharmacy
基金
军队医疗机构制剂标准提高科研专项课题(No.14ZJZ02)
