摘要
目的:比较鲜品和干品高良姜挥发性成分的异同,为其质量评价提供依据。方法:顶空固相微萃取(HS-SPME)结合气相色谱联用质谱法(GC-MS法)分析不同高良姜样品的挥发性成分。具体条件:SPME萃取头于样品瓶中80℃平衡10 min,再吸附20 min,250℃解吸5 min进样;GC分离采用HP-5MS毛细管柱(30 m×0.25 mm×0.25μm),载气为He气,柱流量1.0 mL·min^(-1),柱温为程序升温(初始温度60℃,以4℃·min^(-1)的速率程序升温至116℃,保持20 min,以5℃·min^(-1)的速率程序升温至160℃,以20℃·min^(-1)速率提升至280℃,保持2 min);质谱检测为EI离子源,电子能量70 e V,四极杆温度150℃,接口温度250℃,质量范围为m/z 50~500。结果:依据MS数据库及文献检索,共鉴定出主要化合物30个,主要为烯、醇类成分,其中桉油精、α-松油醇、石竹烯、α-香柑油烯及α-金合欢烯含量较高。新鲜和干燥高良姜挥发性成分的种类差异不大,但含量差异较为明显。干品高良姜中依然含有较高含量的桉油精,与高良姜干品浓烈的辛香味有关。结论:本法能够快速比较鲜/干高良姜中挥发性成分,为高良姜挥发性成分质控指标选择提供依据。
Objective： To analyze and compare the volatile components in fresh and dried Alpinia officinarum.Methods： The volatile components in Alpinia officinarum from Xuwen of Guangdong province was analyzed by using head space-solid phase microextraction （ HS,SPME ） coupled with GC-MS analysis （ HS.SPME,GC.MS ）.The conditions were： SPME fiber （ 100 μm PDMS ） was preconditioned at 80 ℃ for 10 min in the bottle with 50 mg sample, then extracion was perforned for 20 min, desorption was 5 min for injection. GC analysis was performed on HP-5MS capillary column （ 30 m × 0.25 mm × 0.25 μm ） with high purity helium as carrier gas at the flow rate of 1.0 mL· min-1. The column temperature was set as follow： started at 60 ℃ and then 4 ℃ · min-1 to 116 ℃ and held for 20 min, after that 5 ℃ · min-1 to 160 ℃, and finally 20 ℃· min-1 to 280 ℃ and held for 2 min. EI-MS, the ionization energy was 70 eV, the inlet, ionization source temperature were 250 ℃ and 150 ℃, respectively. The scan range was m/z 50-500. The peak identification was based on MS data library and literature search and the content of individual component in each sample was compared. Results： About 30 volatile components in Alpinia officinarum were identified. Eucalyptol, α-terpineol, caryophyllene, α-bergamotene,α-farnesene were the major compounds. The volatile components in fresh and dried Alpinia officinarum were similar, but their contents were obviously different. Conclusion： This is a rapid method for investigation and comparison of volatile components in fresh/dried Alpinia officinarum, which is helpful to select the quality markers for analysis of volatile components in Alpinia officinarum.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2017年第9期1615-1620,共6页
Chinese Journal of Pharmaceutical Analysis
基金
澳门科学技术发展基金项目(FDCT034/2017/A1)
澳门大学研究基金项目(MYRG2015-00122)
