摘要
目的应用Box-Behnken效应面法优化肾茶提取工艺,运用大孔吸附树脂优选肾茶总黄酮的纯化工艺。方法以肾茶总黄酮得率为评价指标,以乙醇体积分数,液料比和提取时间为考察因素,采用Box-Behnken效应面法优化加热回流提取工艺,并进行预测分析。通过静态和动态吸附试验考察大孔吸附树脂对肾茶总黄酮的纯化效果,并研究各因素条件对其纯化效果的影响,筛选主要影响指标的最佳工艺条件。结果肾茶最佳提取工艺为:乙醇体积分数为52.52%,液料比(mL:g)为15:1,提取时间为90分钟。DM-130大孔吸附树脂较适合肾茶总黄酮的纯化,静态吸附生药浓度为0.1 g/mL,药液pH值为2;动态吸附上样量为60 mL,吸附时间为50分钟,除杂水用量为3倍柱体积,洗脱剂为70%乙醇,洗脱剂用量为3倍树脂体积。结论 Box-Behnken效应面法用于优选肾茶的提取工艺是可行的,建立的数学模型和实验观察数据相符,DM-130大孔吸附树脂较适合肾茶总黄酮的纯化。
Objective To optimize extraction- technology of total flavonoids from Clerodendranthus spicatus （Thunb） C. Y. Wu by Box-Behnken design response surface methodology. To establish the purification technology of total flavonoids of Clerodendranthus spicatus. Methods Ethanol content, liquid- solid ratio and extraction time were used to examine the yield of total flavonoids. The Box-Behnken effect surface method was used to optimize the extraction process of heating reflux. The static and dynamic adsorption was used to observe the macroporous adsorption resin purification effect of total flavonoids of Clerodendranthus spicatus. The best purification process conditions were screened out and optimized. Results The best extraction technology：Optimum process parameters were obtained as followings： the ethanol content was 52.52%, liquid-solid ratio was 15 ： 1, extraction duration was 90 min. DM-130 macroporous adsorption resin was suitable for purification of total flavonoids, The static adsorption concentration was 0.1 g/mL, pH was 2; Loading quantity of sample of dynamic adsorption was 60 mL, the adsorption time was 50 minutes. Water usage of edulcoration was three times as much as column volume; elution solvent was 70% ethanol, elution solvent volume was 3 three times as much as resin volume. Conclusion Box-Behnken design for optimization of extraction technology of Clerodendranthus spicatus （ Thunb. ） C. Y. Wu is feasible, The established mathematical model agrees well with the experimental oh-servations. DM-130 is the best kind of macroporous resin for purification of Clerodendranthus spicatus （Thunb.） C. Y. Wu.
作者
蓝伦礼
刘梦楚
邹晓红
曾元儿
曹骋
刘庆飞
李小莹
江滨
LAN Lunli L1U Mengchu ZOU Xiaohong et al. Traditional Chinese medicine analysis laboratory College of traditional Chinese medicine Guangzhou university of Chinese medicine institute Guangzhou 510006
出处
《环球中医药》
CAS
2017年第9期1033-1039,共7页
Global Traditional Chinese Medicine
基金
南药研究协同创新中心专项基金(2011)
关键词
肾茶
总黄酮
BOX-BEHNKEN效应面法
大孔吸附树脂
Clerodendranthus spicatus （Thunb.） C. Y. Wu
General flavone
Box-Behnkendesign response surface methodology
Macroporous resin
