摘要
目的观察Clara细胞分泌蛋白(Clara cell secretory 16-KD protein,CC16)在脓毒症大鼠心肌组织中的表达,探讨其在脓毒症心肌损伤发生机制中的作用。方法将40只健康雄性Wistar大鼠随机分为假手术组(SH组,n=20)及脓毒症组(CLP组,n=20),采用盲肠结扎穿孔法(CLP)制备脓毒症动物模型,24 h后,检测各组的血清脑钠肽(BNP)、肌钙蛋白I(cTnI)的水平,观察心肌组织病理变化及心肌组织CC16的表达水平。结果CLP组出现明显的心肌损伤,CLP组血清BNP、cTnI高于SH组(P<0.01),心肌CC16低于SH组(P<0.01)。结论 CC16在脓毒症大鼠心肌组织中表达下降,CC16可能在脓毒症心肌损伤发病机制中起重要作用。
Objective To investigate the expression of CC16 in myocardial tissues in septic rats,and its role in sepsis-induced myocardial injury in rats. Methods Forty healthy male Wistar rats were randomly divided into sham group（ SH group,n = 20） and sepsis group（ CLP group,n = 20）. The sepsis models were established by cecal ligation and puncture（ CLP）. The levels of Serum brain natriuretic peptide（ BNP） and cardiac troponin I（ cTnI） were measured24 hours after CLP. The myocardial tissue pathological changes and the expression of CC16 in myocardial tissue were observed. Results In CLP group,myocardial tissues were seriously injured,the levels of Serum BNP and cTnI were significantly higher than SH group（ P〈0. 01）,and the level of myocardial CC16 was significantly lower than SH group（ P〈0. 01）. Conclusion The expression of CC16 decreases in myocardial tissues in septic rats,CC16 may play an important role in the pathogenesis of sepsis-induced myocardial injury.
作者
郑晓丽
陈芳
张小乔
刘伯毅
ZHENG Xiao - li CHEN Fang ZHANG Xiao - qiao LIU Bo- yi.(Department of Geriatric Medicine ＆ General Medicine, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, Hubei, Chin)
出处
《广东医学》
CAS
北大核心
2017年第17期2598-2600,共3页
Guangdong Medical Journal
基金
十堰市科技局指导项目(编号:2014Y17)
