摘要
以野生宁前胡根、茎、叶为外植体对其愈伤组织诱导进行了初步研究,以期为野生宁前胡高效快速繁殖和种质资源保护提供依据。结果表明:叶片愈伤组织诱导最佳条件为NAA 0.5mg/L+TDZ 0.05mg/L,最高诱导率为95.56%,黑暗或低光照度以及叶片远轴面接触培养基有利于叶片愈伤组织的形成,岀愈最早时间为8d;茎段愈伤组织诱导最佳条件为NAA 0.5mg/L+6-BA 2.0mg/L,最高诱导率达80.00%;根愈伤组织诱导最佳条件为NAA 0.5mg/L+6-BA 2.0mg/L或2,4-D 2.0mg/L+KT 0.5mg/L,最高诱导率达78.89%。
The study in this paper aimed to provide the protocol for rapid propagation and germplasm resources conservation of wild Peucedanum praeruptorum Dunn, using root, stem and leaf as explants for callus induction.Results：The optimum condition for leaf callus induction was NAA 0.5 mg/L＋ TDZ 0.05 mg/L,the highest induction rate up.to 95.56 %, darkness or low light intensity and abaxial surface of leaf laid up on the medium was propitious to callus formation,8 days was the earliest time for callus induction.The optimum condition for stem callus inducement was NAA 0.5 mg/L＋6-BA 2 mg/L,the highest induction rate was 80.00%.While NAA 0.5 mg/L＋6-BA 2 mg/L or 2,4-D 2 mg/L ＋KT 0.5 mg/L was optimal for root callus induction,the highest induction rate reached 78.89%.
出处
《种子》
北大核心
2017年第9期1-5,共5页
Seed
基金
国家青年自然科学基金(31600254)
江苏省青年自然科学基金(BK 20150491)
中医药行业专项(201407003)
江苏省高校优势学科资助项目(苏政办[2014]37号)
关键词
宁前胡
白花前胡
愈伤组织
诱导
ning qianhu
Peucedanum praeruptorum Dunn
callus
inducement
